RT-qPCR

Comparative evaluation of total RNA extraction methods in Theobroma cacao using shoot apical meristems

D. V. Silva, Branco, S. M. J., Holanda, I. S. A., Royaert, S., Motamayor, J. C., Marelli, J. P., Corrêa, R. X., Silva, D. V., Branco, S. M. J., Holanda, I. S. A., Royaert, S., Motamayor, J. C., Marelli, J. P., and Corrêa, R. X., Comparative evaluation of total RNA extraction methods in Theobroma cacao using shoot apical meristems, vol. 15, p. -, 2016.

Theobroma cacao is a species of great economic importance with its beans used for chocolate production. The tree has been a target of various molecular studies. It contains many polyphenols, which complicate the extraction of nucleic acids with the extraction protocols requiring a large amount of plant material. These issues, therefore, necessitate the optimization of the protocols. The aim of the present study was to evaluate different methods for extraction of total RNA from shoot apical meristems of T.

Genes related to high temperature tolerance during maize seed germination

S. M. F. Dutra, Von Pinho, E. V. R., Santos, H. O., Lima, A. C., Von Pinho, R. G., and Carvalho, M. L. M., Genes related to high temperature tolerance during maize seed germination, vol. 14, pp. 18047-18058, 2015.

The identification of genes related to heat tolerance is fundamental for the development of high-quality seeds that are tolerant to heat stress condition. The objective of this study was to evaluate maize lineages and the gene expression involved in high temperature tolerance during germination using physiological tests, proteomics, and transcriptome analysis. Seeds from six maize lineages (30, 44, 54, 63, 64, and 91) with different levels of tolerance to high temperatures were used. Lineages 54 and 91 were observed to be more tolerant to high temperature conditions.

Effect of DNase treatment on RNA extraction from preimplantation murine embryos

J. Norhazlin, Nor-Ashikin, M. N. K., Hoh, B. P., Kadir, S. H. Sheikh Abd, Norita, S., Mohd-Fazirul, M., Wan-Hafizah, W. J., Razif, D., Rajikin, M. H., and Abdullah, B., Effect of DNase treatment on RNA extraction from preimplantation murine embryos, vol. 14, pp. 10172-10184, 2015.

The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation embryos at different stages of development. The quality of the samples was confirmed using a microarray and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) analysis.

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