Ryanodine receptor protein

A new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle

A. A. Droval, Binneck, E., Marin, S. R. R., Paião, F. G., Oba, A., Nepomuceno, A. L., and Shimokomaki, M., A new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle, vol. 11, pp. 821-829, 2012.

Some genes affect meat quality in chickens. We looked for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could be associated with PSE (pale, soft and exudative) meat. Because RyR genes are over 100,000 bp long and code for proteins with about 5000 amino acids, primers were designed to amplify a fragment of hotspot region 2, a region with a high density of mutations in other species. Total blood DNA was extracted from 50 birds, 25 that had PSE meat and 25 normal chickens.

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