Salmonella paratyphi A flagellin

Separation, purification, and identification of flagellin, and preparation of its antisera

M. D. Hu, Wang, G. S., Xu, J., Yao, W., He, B. F., Yang, Y., Mao, M., Wang, Q., and Xu, J. C., Separation, purification, and identification of flagellin, and preparation of its antisera, vol. 13, pp. 9161-9170, 2014.

The aim of this study was to separate, purify, and identify Salmonella paratyphi A flagellin, and to prepare its antisera. Primary flagellin was isolated from S. paratyphi A using the acid lysis method. The flagellin was purified with weak anion exchange chromatography and the protein was identified with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, and negative staining with phosphotungstic acid with scanning electron microscopy (SEM). The production of the obtained flagellin was then quantified.

Subscribe to Salmonella paratyphi A flagellin