Sequence characterization

Molecular cloning and characterization of a novel chicken gene named grni

Y. Hua, Hui, D. Y., Rong, Z. H., and Cheng, T., Molecular cloning and characterization of a novel chicken gene named grni, vol. 13, pp. 386-395, 2014.

The full-length cDNA sequence of a novel expressed sequence tag (GenBank accession No. HQ184338) that was differentially expressed during Newcastle disease virus (NDV) infection in chickens was cloned from the chicken spleen by a rapid amplification of cDNA ends assay. This gene was further analyzed using bioinformatic methods and named grni. The full-length cDNA sequence was 1698 bp without introns, locating between 104,691,934 and 104,693,618 in galGal4 on chromosome 2. The open reading frame (ORF) contained 261 bp and encoded a deduced protein of 86 amino acid residues.

Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility

M. H. Deng, Wen, J. F., Huo, J. L., Zhu, H. S., Dai, X. Z., Zhang, Z. Q., Zhou, H., and Zou, X. X., Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility, vol. 11, pp. 3020-3031, 2012.

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs.

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