Sequence-characterized amplified region

Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst

M. A. Khan, Cheng, J. L., Mei, Z. Q., Wei, C. L., Fu, J. J., Khan, M. A., Cheng, J. L., Mei, Z. Q., Wei, C. L., and Fu, J. J., Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst, vol. 15, p. -, 2016.

Development of sequence-characterized amplified region (SCAR) markers from random-amplified polymorphic DNA (RAPD) fragments is a valuable molecular approach for the genetic identification of different species. By using SCAR markers, molecular analysis is reduced to a simple polymerase chain reaction (PCR) analysis using primers designed from the amplicon sequence of RAPD.

Sex differential marker FD for rapid sex identification of Litsea cubeba

Q. Wu, Chen, Y., Wang, Y., and Lin, L., Sex differential marker FD for rapid sex identification of Litsea cubeba, vol. 14, pp. 12820-12827, 2015.

Litsea cubeba is an important economic tree in China. Sex identification of the species is required to reduce breeding costs. Molecular biology is an ideal method to achieve this aim because of the lack of morphological differences between male and female plants, especially at the seedling stage. Sequence-related amplified polymorphism was used to amplify sex-related bands. Following sequencing, the amplified fragment Dwas used to create a sequence-characterized amplified region (SCAR) marker, FD.

Molecular authentication of Gynostemma pentaphyllum through development and application of random amplification polymorphic DNA sequence-characterized amplified region marker

J. Zhou, Wu, Y. S., Zhao, R. Q., Jiang, J. F., Luo, Y., Ma, C. T., and Qian, J. Y., Molecular authentication of Gynostemma pentaphyllum through development and application of random amplification polymorphic DNA sequence-characterized amplified region marker, vol. 14, pp. 16204-16214, 2015.

Due to the morphological similarities of aerial parts, it is difficult to distinguish Gynostemma pentaphyllum from Cayratia japonica, which is usually an adulterant of the former. To develop a reliable method for the identification and authentication of G. pentaphyllum, a combination of random amplification polymorphic DNA (RAPD) technique with sequence-characterized amplified region (SCAR) markers was studied. Twenty-five samples of G. pentaphyllum and two samples of C.

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