Shotgun library

Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)

M. P. C. Telles, Peixoto, F. P., Lima, J. S., Resende, L. V., Vianello, R. P., Walter, M. E. M. T., and Collevatti, R. G., Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae), vol. 10, pp. 321-325, 2011.

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local populations were genotyped using the 12 primers designed to analyze the polymorphisms at each locus. The number of alleles per locus ranged from one to six; two loci were monomorphic.

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