Shotgun library

Isolation and characterization of microsatellite markers in the armored catfish Hypostomus gymnorhynchus (Loricariidae)

M. P. C. Telles, Resende, L. V., Brondani, R. P. V., Collevatti, R. G., Costa, M. C., and Júnior, N. J. Silva, Isolation and characterization of microsatellite markers in the armored catfish Hypostomus gymnorhynchus (Loricariidae), vol. 9. pp. 1770-1774, 2010.

We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each locus. All loci showed low polymorphism, with a low number of alleles per locus (1 or 2), except locus Hg_E19, which had 11 alleles.

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