shRNA

Hypoxia-inducible factor-2α (HIF-2α) mediates the effects of hypoxia on the promotion of HeLa cell viability, colony formation, and invasion capacity in vitro

J. Xiong, Zhu, F. F., and Nie, M. F., Hypoxia-inducible factor-2α (HIF-2α) mediates the effects of hypoxia on the promotion of HeLa cell viability, colony formation, and invasion capacity in vitro, vol. 14, pp. 3281-3292, 2015.

Hypoxia reduces the oxygen supply to tumor cells and may limit tumor cell growth. However, hypoxia promotes tumor cell metabolic adaptation, apoptosis resistance, angiogenesis, invasion, and metastasis. Hypoxia-inducible factor-2α (HIF-2α) may be responsible for these hypoxia-induced changes. In this study, we investigated the effects of hypoxia and HIF-2α knockdown in HeLa cells. HIF-2α shRNA lentivirus was used to knock down HIF-2α expression; cell viability, colony formation, invasion capacity, and gene expression were assessed.

Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi

N. Ally, Zou, X. L., Jiang, B. C., Qin, L., Zhai, L., Xiao, P., and Liu, H. L., Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi, vol. 11, pp. 4019-4033, 2012.

Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonucleotides targeting the coding sequence of mouse VEGFA mRNA and one negative control (shNC) were designed and cloned into a pGPU6/GFP/Neo siRNA expression vector, and transiently transfected into MGC.

Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

S. Wei, Zan, L. S., Wang, H. B., Cheng, G., Du, M., Jiang, Z., Hausman, G. J., McFarland, D. C., and Dodson, M. V., Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes, vol. 12, pp. 494-505, 2013.

Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine.

Subscribe to shRNA