Silkworm

Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers

G. Q. Wei, Yu, L., Liu, C. L., Zhu, B. J., and Ding, H. J., “Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers”, vol. 12, pp. 2351-2359, 2013.

In the silkworm, Bombyx mori, normal markings are mainly controlled by the +P gene, which is located on the second chromosome. Due to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the +P gene based on an SSR linkage map using silkworm strains P50 and H9, which are normal marking and sex-limited marking, respectively. The +P gene was found to be linked to 3 SSR markers.

Allelic variability in the third intron of the fibroin light chain gene in Bombyx mori (Lepidoptera: Bombycidae)

J. F. Barbosa, Bravo, J. P., Zanatta, D. B., Silva, J. L. C., and Fernandez, M. A., “Allelic variability in the third intron of the fibroin light chain gene in Bombyx mori (Lepidoptera: Bombycidae)”, vol. 8, pp. 197-206, 2009.

Conformation-sensitive gel electrophoresis is a useful method for identifying allele polymorphism; it provides co-dominant molecular markers. Using this method, we identified genetic variability in the third intron of the fibroin light chain gene, fib-L, in six Bombyx mori strains. Only Chinese C21A strain did not demonstrate allelic alterations, showing only homoduplex DNA molecules. We found distinct heteroduplex profiles in the Japanese HAA, M12B and M19-2 and the Chinese C25B and C24-2 strains.

Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

R. M. C. Brancalhão, Torquato, E. F. B., and Fernandez, M. A., “Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus”, vol. 8, pp. 162-172, 2009.

A multiple nucleopolyhedrovirus previously isolated from infected Bombyx mori L. larvae (BmMNPV) in Paraná state, Brazil, was inoculated into B. mori larvae to examine susceptibility and cytopathology in silk gland cells. The anterior, middle and posterior silk glands were removed from the infected silkworm at different times post-inoculation and processed for cytopathology studies by light and transmission electron microscopy. BmMNPV infection was only detected at 72 h post-inoculation in cells of the middle and posterior silk glands.

Molecular markers in commercial Bombyx mori (Lepidoptera: Bombycidae) hybrids susceptible to multiple nucleopolyhedrovirus

L. F. C. Ribeiro, Zanatta, D. B., Bravo, J. P., Brancalhão, R. M. C., and Fernandez, M. A., “Molecular markers in commercial Bombyx mori (Lepidoptera: Bombycidae) hybrids susceptible to multiple nucleopolyhedrovirus”, vol. 8, pp. 144-153, 2009.

The silkworm Bombyx mori L. is particularly susceptible to virus diseases, especially B. mori nucleopolyhedrovirus (BmNPV). Disease resistance, along with high productivity, are important selection criteria for developing commercial hybrids of B. mori. We used bioassays and molecular markers linked to susceptibility/resistance to baculovirus infection to analyze the response of commercial B. mori hybrids from two companies to a geographic isolate of B. mori multiple nucleopolyhedrovirus (BmMNPV) from Paraná state in Brazil.

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