Simple sequence repeat Genomic in situ hybridization

Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance

M. Lu, Wang, L., Zhang, J., Sun, S., Li, Y., Du, W., Wu, J., Zhao, J., Yang, Q., and Chen, X., “Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance”, vol. 13, pp. 10678-10689, 2014.

A wheat germplasm line 13-2-2 with resistance to powdery mildew was isolated; this line was derived from common wheat cv. W770B and rye, Secale cereale L. (2n = 2x = 14, RR). The line was characterized based on cytological, genomic in situ hybridization (GISH), sequence-characterized amplified region (SCAR), and simple sequence repeat (SSR) analyses. The mitotic and meiotic investigations showed that the chromosome number and configuration of 13-2-2 were 2n = 42 = 21 II.

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