Simple sequence repeat markers

De novo assembly and characterization of the root transcriptome and development of simple sequence repeat markers in Paphiopedilum concolor

D. M. Li, Zhao, C. Y., Liu, X. R., Liu, X. F., Lin, Y. J., Liu, J. W., Chen, H. M., and Lǚ, F. B., “De novo assembly and characterization of the root transcriptome and development of simple sequence repeat markers in Paphiopedilum concolor”, vol. 14, pp. 6189-6201, 2015.

Paphiopedilum orchids (Orchidaceae) have attracted much attention from botanists and horticulturists because of their peculiar leaves and beautiful flowers. Furthermore, the dry roots of Paphiopedilum plants have well-known medicinal uses. However, it is unknown how sensitive and plastic the root genes are to environmental changes or how these environmental changes regulate the biosynthesis of active ingredients. In this study, we chose Paphiopedilum concolor for root sequencing, as it is widely used as a parent in breeding experiments.

Genetic diversity in elite inbred lines of maize and its association with heterosis

E. H. Fernandes, Schuster, I., Scapim, C. A., Vieira, E. S. N., and Coan, M. M. D., “Genetic diversity in elite inbred lines of maize and its association with heterosis”, vol. 14, pp. 6509-6517, 2015.

The objective of the current study was to apply molecular markers (microsatellites) in the analysis of genetic diversity of 48 lines of the elite maize germplasm stored in the bank of the Cooperativa Central de Pesquisa Agrícola - Coodetec, PR, Brazil, and estimate the correlation between genetic distance and heterosis and hybrid performance from the crosses among these maize lines. Forty-four random primers were used and amplification of 124 polymorphic fragments was obtained.

Genetic divergence among sweet corn lines estimated by microsatellite markers

A. D. Lopes, Scapim, C. A., Mangolin, C. A., and Machado, M. F. P. S., “Genetic divergence among sweet corn lines estimated by microsatellite markers”, vol. 13, pp. 10415-10426, 2014.

The purpose of this study was to analyze the genetic diversity of 15 sugary-1 sweet corn lines by microsatellite markers. One hundred pairs of simple sequence repeat primers that were mapped for field corn were tested. Of these primers, 15% were polymorphic, and all were selected for the evaluation. These primers identified a total of 39 alleles among the 15 loci that were evaluated.

Genetic identification of Theobroma cacao L. trees with high Criollo ancestry in Soconusco, Chiapas, Mexico

J. A. Vázquez-Ovando, Molina-Freaner, F., Nuñez-Farfán, J., Ovando-Medina, I., and Salvador-Figueroa, M., “Genetic identification of Theobroma cacao L. trees with high Criollo ancestry in Soconusco, Chiapas, Mexico”, vol. 13, pp. 10404-10414, 2014.

Criollo-type cacao trees are an important pool of genes with potential to be used in cacao breeding and selection programs. For that reason, we assessed the diversity and population structure of Criollo-type trees (108 cultivars with Criollo phenotypic characteristics and 10 Criollo references) using 12 simple sequence repeat (SSR) markers. Cultivars were selected from 7 demes in the Soconusco region of southern Mexico. SSRs amplified 74 alleles with an average of 3.6 alleles per population.

Heterotic groups in tropical maize germplasm by test crosses and simple sequence repeat markers

C. G. Aguiar, Schuster, I., Júnior, A. T. Amaral, Scapim, C. A., and Vieira, E. S. N., “Heterotic groups in tropical maize germplasm by test crosses and simple sequence repeat markers”, vol. 7, pp. 1233-1244, 2008.

The objectives of the present study were to determine heterotic groups of germplasm lines of tropical maize by test crosses and by simple sequence repeat (SSR) markers and to compare five grouping methods of heterogeneous maize. Sixteen lines of nine populations in the S5 generation were evaluated in test crosses with three testers.

SSR analysis demonstrates that olive production in the southern Marmara region in Turkey uses a single genotype

A. Ipek, Barut, E., Gulen, H., Oz, A. T., Tangu, N. A., and Ipek, M., “SSR analysis demonstrates that olive production in the southern Marmara region in Turkey uses a single genotype”, vol. 8, pp. 1264-1272, 2009.

The southern Marmara region in Turkey was surveyed to determine the olive cultivars that are used for olive production. Genetic diversity analysis using simple sequence repeat (SSR) markers indicated that the cultivar Gemlik is the major olive cultivar grown in this region, while other olive cultivars are grown only for use as pollinators of Gemlik or for growers’ own exotic consumption. Although the quality of Gemlik is widely accepted in Turkey, its tendency toward alternate bearing is a major drawback.

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