Simple sequence repeats

Development, characterization, and annotation of potential simple sequence repeats by transcriptome sequencing in pears (Pyrus pyrifolia Nakai)

H. Zhou, Cai, B. H., Lu, Z. Q., Gao, Z. H., Qiao, Y. S., Zhou, H., Cai, B. H., Lu, Z. Q., Gao, Z. H., and Qiao, Y. S., Development, characterization, and annotation of potential simple sequence repeats by transcriptome sequencing in pears (Pyrus pyrifolia Nakai), vol. 15, p. -, 2016.

Simple sequence repeats (SSRs), one of the most powerful molecular markers, can be used for DNA fingerprinting, variety identification, genetic mapping, and marker-assisted selection. Using the pear’s (Pyrus pyrifolia Nakai) 75,764 unigenes (55,676,271 bp) obtained by deep transcriptome sequencing, a total of 10,622 novel SSRs were identified in 9154 unigenes, accounting for 14.02% of all unigenes. The average length and distribution of these SSRs was about 16 bp and 5.24 kb, respectively.

Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.)

Z. J. Yang, Peng, Z. S., Yang, H., Yang, Z. J., Peng, Z. S., and Yang, H., Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.), vol. 15, p. -, 2016.

Simple sequence repeats (SSRs) are highly informative, polymorphic, and co-dominant Mendelian markers that provide an important genomic resource for genetic research. Recently, the use of large-scale transcriptome sequence has become a reliable and efficient approach for the identification and development of new EST-SSR markers. In this study, 8389 potential SSRs with a minimum of five repetitions for all motifs were identified from 121,210 unigenes.

Subscribe to Simple sequence repeats