Sleeping Beauty system

Application of the Sleeping Beauty system in Saanen goat fibroblast cells for establishing persistent transgene expression

B. C. Jiang, Kaleri, H. A., Zhang, H. X., Chen, J., and Liu, H. L., Application of the Sleeping Beauty system in Saanen goat fibroblast cells for establishing persistent transgene expression, vol. 10, pp. 3347-3355, 2011.

The Sleeping Beauty (SB) transposon system is a promising new method for establishing persistent transgene expression in vivo. We applied the SB system for enhancing transgenesis in Saanen dairy goat fibroblast cells. We constructed a pKT2/CMV-EGFP-IRES-PURO vector and investigated the influence of transposon and transposase vector ratios on transfection efficiency in the Saanen goat fibroblast cells. To enhance the SB system performance, we developed a new transfection technique (double-transfection method) for the SB system.

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