Somatic cell nuclear transfer

6-Dimethylaminopurine and cyclohexamide are mutagenic and alter reproductive performance and intrauterine development in vivo

R. J. Oliveira, Mantovani, M. S., Pesarini, J. R., Mauro, M. O., da Silva, A. F., Souza, T. R., and Ribeiro, L. R., 6-Dimethylaminopurine and cyclohexamide are mutagenic and alter reproductive performance and intrauterine development in vivo, vol. 14, pp. 834-849, 2015.

The compounds 6-dimethylaminopurine (6-DMAP) and cyclohexamide (CHX) are currently used to stimulate the development of embryos produced by nuclear transfer in the production of cloned mammals with a great deal success. This study investigated the effects of 6-DMAP and CHX in vivo using biological assays to evaluate reproductive performance in females, teratogenesis, and mutagenesis.

In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence

R. P. C. Gerger, Ribeiro, E. S., Forell, F., Bertolini, L. R., Rodrigues, J. L., Ambrósio, C. E., Miglino, M. A., Mezzalira, A., and Bertolini, M., In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence, vol. 9, pp. 295-302, 2010.

The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence levels on in vitro development of cloned bovine embryos. In vitro-matured bovine oocytes were manually bisected and selected by DNA staining. One or two enucleated hemi-cytoplasts were paired and fused with an adult skin somatic cell.

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