SOX2

Cryopreservation does not alter karyotype, multipotency, or NANOG/SOX2 gene expression of amniotic fluid mesenchymal stem cells

P. C. Ângelo, Ferreira, A. C. S., Fonseca, V. D., Frade, S. P., Ferreira, C. S., Malta, F. S. V., Pereira, A. K., Leite, H. V., Brum, A. P., Pardini, V. C., Gomes, K. B., and Cabral, A. C. V., Cryopreservation does not alter karyotype, multipotency, or NANOG/SOX2 gene expression of amniotic fluid mesenchymal stem cells, vol. 11, pp. 1002-1012, 2012.

Cryopreservation of mesenchymal stem cells from amniotic fluid is of clinical importance, as these cells can be harvested during the prenatal period and stored for use in treatments. We examined the behavior of mesenchymal stem cells from human amniotic fluid in culture that had been subjected to cryopreservation. We assessed chromosomal stability through karyotype analysis, determined whether multipotent capacity (differentiation into adipogenic, chondrogenic, and osteogenic cells) is maintained, and analyzed SOX2 and NANOG expression after thawing.

Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones

D. Drakulic, Krstic, A., and Stevanovic, M., Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones, vol. 11, pp. 1385-1400, 2012.

SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and characterized SOX2-overexpressing NT2/D1 cell clones.

Genetic diversity of stem cells and their functional impact on the development of neural tube defects in Eastern population of India

A. K. Saxena, Pandey, S., and Pandey, L. K., Genetic diversity of stem cells and their functional impact on the development of neural tube defects in Eastern population of India, vol. 12, pp. 2380-2390, 2013.

Anencephaly and myelomeningocele are the 2 most common forms of neural tube defects (NTDs). During embryogenesis large numbers of extrinsic and intrinsic factors are responsible for the closing of the neural tube. "Stem cells" maintain the pluripotency during differentiation of 3 germ layers, including the neural ectoderm. We examined the role of Oct4, Nanog3, and Sox2 genes in the etiopathology of NTDs in an eastern Indian population using PCR-based DNA analysis. The highest frequency (16%) of complete loss of the Sox2 gene was found in NTDs.

Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Y. X. Fan, Gu, C. H., Zhang, Y. L., Zhong, B. S., Wang, L. Z., Zhou, Z. R., Wang, Z. Y., Jia, R. X., and Wang, F., Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine, vol. 12, pp. 6067-6079, 2013.

Mesenchymal stem cells derived from bone marrow (BMSCs) are a population of self-renewing multipotent cells that are capable of differentiating into various cellular lineages, and are widely employed in tissue engineering and cell therapy. Recently, clinical research involving BMSCs has become increasingly popular. In order to conduct appropriate research, it is first necessary to amplify large amounts of functional BMSCs in vitro. However, after several passages of expanding in vitro, the proliferation and differentiation potential of BMSCs gradually decline.

Subscribe to SOX2