Spermadhesin

Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA

J. B. Cajazeiras, Melo, L. M., Albuquerque, E. S., Rádis-Baptista, G., Cavada, B. S., and Freitas, V. J. F., Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA, vol. 8, pp. 1147-1157, 2009.

Low purification efficiency and incomplete characteriza­tion of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion protein in Escherichia coli Top10 cells. Recombinant clones were se­lected by growth in ampicillin-enriched medium, PCR amplification and nucleotide sequencing.

Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma

D. Ítalo Alv Teixeira, Melo, L. Magalhães, Gadelha, C. Alberto de, da Cunha, R. Maranguape, Bloch, Jr., C., Rádis-Baptista, G., Cavada, B. Sousa, and Freitas, V. José de F., Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma, vol. 5, pp. 79-87, 2006.

Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-exchange chromatography to isolate this seminal protein. Semen was obtained from four adult Saanen bucks. Seminal plasma was pooled, dialyzed against distilled water and freeze-dried.

Subscribe to Spermadhesin