Sprague Dawley rats

Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate

X. K. Xing, Feng, H. G., Yuan, Z. Q., Xing, X. K., Feng, H. G., and Yuan, Z. Q., Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate, vol. 15, p. -, 2016.

This study investigated the efficacy and feasibility of inducing the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro using Sprague Dawley rats, as a model of hepatocyte generation for cell transplantation. BMSCs were isolated and grown using the adherent method and exposed to 5 or 10% liver tissue homogenate, before being collected for analysis after 0, 7, 14, and 21 days. Immunofluorescence and western blotting were employed to detect the liver-specific markers a-fetoprotein (AFP) and albumin (ALB).

Subscribe to Sprague Dawley rats