SRAP

Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.

S. H. Wang, Li, Y., Li, Z. Q., Chang, L., and Li, L., “Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.”, vol. 14, pp. 2015-2022, 2015.

Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequence-related amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify.

Genetic diversity and population structure of Eucommia ulmoides Oliver, an endangered medicinal plant in China

J. Yu, Wang, Y., Peng, L., Ru, M., and Liang, Z. S., “Genetic diversity and population structure of Eucommia ulmoides Oliver, an endangered medicinal plant in China”, vol. 14, pp. 2471-2483, 2015.

Eucommia ulmoides Oliver, one of the tertiary relict species found only in China, is the only extant species of Eucommiaceae. Using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers, we studied the genetic diversity and population genetic structure of 187 accessions from 17 E. ulmoides populations throughout its main distribution in China. A total of 65 bands were amplified using eight ISSR primers, of which 50 bands (76.9%) were polymorphic.

Genetic diversity and population structure of Stipa bungeana, an endemic species in Loess Plateau of China, revealed using combined ISSR and SRAP markers

J. Yu, Jing, Z. B., and Cheng, J. M., “Genetic diversity and population structure of Stipa bungeana, an endemic species in Loess Plateau of China, revealed using combined ISSR and SRAP markers”, vol. 13, pp. 1097-1108, 2014.

Sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among 15 natural populations of Stipa bungeana from the Loess Plateau of China. Using 15 SRAP primers, 504 (99.80%) polymorphic loci were detected, and 372 polymorphic loci (96.12%) were identified using 15 ISSR primers. At the species level, the S. bungeana populations showed relatively low levels of genetic diversity (H_E = 0.2017 for SRAP; H_E = 0.2066 for ISSR).

Genetic diversity and population structure in Harpadon nehereus based on sequence-related amplified polymorphism markers

Z. H. Zhu, Li, H. Y., Qin, Y., and Wang, R. X., “Genetic diversity and population structure in Harpadon nehereus based on sequence-related amplified polymorphism markers”, vol. 13, pp. 5974-5981, 2014.

In this study, the genetic diversity among ten populations of the Bombay duck was studied on the basis of sequence-related amplified polymorphism (SRAP). The ten populations were collected from the East China Sea and South China Sea areas. A total of 98 loci were obtained from 292 individuals using eight SRAP primers. The average proportion of polymorphic loci, genetic diversity (H), and Shannon’s information index were 75.20%, 0.2478, and 0.3735, respectively.

SRAP analysis of genetic diversity of nine native populations of wild sugarcane, Saccharum spontaneum, from Sichuan, China

D. Chang, Yang, F. Y., Yan, J. J., Wu, Y. Q., Bai, S. Q., Liang, X. Z., Zhang, Y. W., and Gan, Y. M., “SRAP analysis of genetic diversity of nine native populations of wild sugarcane, Saccharum spontaneum, from Sichuan, China”, vol. 11, pp. 1245-1253, 2012.

Saccharum spontaneum is a wild sugarcane species that is native to and widely distributed in China. It has been extensively used in sugarcane breeding programs, and is being tested for the development of bioenergy cultivars. In order to provide basic information for the exploitation of this species, we analyzed genetic variation among and within native S. spontaneum populations collected from Sichuan, China. Eighty plants from nine native populations were sampled.

Molecular diversity and population structure of the forage grass Hemarthria compressa (Poaceae) in south China based on SRAP markers

L. - K. Huang, Zhang, X. - Q., Xie, W. - G., Zhang, J., Cheng, L., and Yan, H. D., “Molecular diversity and population structure of the forage grass Hemarthria compressa (Poaceae) in south China based on SRAP markers”, vol. 11, pp. 2441-2450, 2012.

Hemarthria compressa is one of the most important and widely utilized forage crops in south China, owing to its high forage yield and capability of adaptation to hot and humid conditions. We examined the population structure and genetic variation within and among 12 populations of H. compressa in south China using sequence-related amplified polymorphism (SRAP) markers. High genetic diversity was found in these samples [percentage polymorphic bands (PPB) = 82.21%, Shannon’s diversity index (I) = 0.352].

Genetic diversity of endangered Polyporus umbellatus from China assessed using a sequence-related amplified polymorphism technique

Y. Zhang, Kang, Y., Qin, Y., Zhou, Z., Lei, M., and Guo, H., “Genetic diversity of endangered Polyporus umbellatus from China assessed using a sequence-related amplified polymorphism technique”, vol. 11. pp. 4121-4129, 2012.

Polyporus umbellatus (Pers.) Fries is an endangered medicinal fungus in China with in vivo anticancer activity, but its genetic information is lacking. Eight natural P. umbellatus strains collected from 7 provinces in China were subjected to sequence-related amplified polymorphism markers to estimate the level and pattern of genetic diversity. Forty-nine primer combinations generated 1219 highly reproducible and discernible loci, among which 1023 were polymorphic. The percentage of polymorphism varied from 35.71 to 96.30 with an average of 83.92.

Assessing genetic divergence in interspecific hybrids of Aechmea gomosepala and A. recurvata var. recurvata using inflorescence characteristics and sequence-related amplified polymorphism markers

F. Zhang, Ge, Y. Y., Wang, W. Y., Shen, X. L., and Yu, X. Y., “Assessing genetic divergence in interspecific hybrids of Aechmea gomosepala and A. recurvata var. recurvata using inflorescence characteristics and sequence-related amplified polymorphism markers”, vol. 11, pp. 4169-4178, 2012.

Conventional hybridization and selection techniques have aided the development of new ornamental crop cultivars. However, little information is available on the genetic divergence of bromeliad hybrids. In the present study, we investigated the genetic variability in interspecific hybrids of Aechmea gomosepala and A. recurvata var. recurvata using inflorescence characteristics and sequence-related amplified polymorphism (SRAP) markers.

A protocol for high-quality genomic DNA extraction from legumes

I. O. Agbagwa, Datta, S., Patil, P. G., Singh, P., and Nadarajan, N., “A protocol for high-quality genomic DNA extraction from legumes”, vol. 11, pp. 4632-4639, 2012.

Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding liquid nitrogen usage and lyophilization. DNA was extracted directly from freshly harvested leaves ground in pre-heated CTAB buffer.

Analysis of genetic diversity among Chinese wild Vitis species revealed with SSR and SRAP markers

Z. B. Jing, Wang, X. P., and Cheng, J. M., “Analysis of genetic diversity among Chinese wild Vitis species revealed with SSR and SRAP markers”, vol. 12, pp. 1962-1973, 2013.

The genetic diversity among 80 Vitis materials including 62 indigenous accessions of 17 wild Vitis species in China and 7 interspecific hybrids, 10 V. vinifera L. cultivars, and 1 V. riparia Michaux were evaluated by simple sequence repeat and sequence-related amplified polymorphism markers. A total of 10 simple sequence repeat primers and 11 sequence-related amplified polymorphism primer combinations were amplified, and 260 bands were generated, of which 252 were polymorphic with an average polymorphism rate of 97.02%.