SSR markers

Taxonomic status of Pinus henryi using multiplexed microsatellite markers

Q. Xie, Liu, Z. - H., and Li, Z. - Q., “Taxonomic status of Pinus henryi using multiplexed microsatellite markers”, vol. 14, pp. 4549-4556, 2015.

The taxonomic status of Pinus henryi, a rare species endemic to China, is still ambiguous. In this study, the genetic relationships among P. henryi and its congeners (P. tabulaeformis, P. tabulaeformis var. mukdensis, and P. massoniana) were revealed using multiplexed microsatellite markers, including chloroplast microsatellites, nuclear microsatellites, and expressed sequence tag microsatellites. The results refute the hypothesis that P. henryi is a subspecies of P. tabulaeformis or P.

Discrimination of common bean cultivars using multiplexed microsatellite markers

P. C. B. Cardoso, Brondani, C., Menezes, I. P. P., Valdisser, P. A. M. R., Borba, T. C. O., Del Peloso, M. J., and Vianello, R. P., “Discrimination of common bean cultivars using multiplexed microsatellite markers”, vol. 13, pp. 1964-1978, 2014.

Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized.

Genetic variation in and spatial structure of natural populations of Dipterocarpus alatus (Dipterocarpaceae) determined using single sequence repeat markers

N. M. Tam, Duy, V. D., Duc, N. M., Giap, V. D., and Xuan, B. T. T., “Genetic variation in and spatial structure of natural populations of Dipterocarpus alatus (Dipterocarpaceae) determined using single sequence repeat markers”, vol. 13, pp. 5378-5386, 2014.

Dipterocarpus alatus (Dipterocarpaceae) is widely distributed in lowland forests in central and southern Vietnam, Cambodia, Laos, Myanmar, Philippines, Thailand, and India. Due to over-exploitation and habitat destruction, the species is now threatened. The genetic variation within and among populations of D. alatus was investigated on the basis of 9 microsatellite (single sequence repeat, SSR) loci. In all, 268 sampled trees from 10 populations in central and southern Vietnam were analyzed in this study.

Development of microsatellite markers of vandaceous orchids for species and variety identification

S. Peyachoknagul, Nettuwakul, C., Phuekvilai, P., Wannapinpong, S., and Srikulnath, K., “Development of microsatellite markers of vandaceous orchids for species and variety identification”, vol. 13, pp. 5441-5445, 2014.

Vandaceous orchids are a group of orchid genera in the subfamily Vandoideae. Among this group, Mokara, Phalaenopsis, and Vanda are the most popular and commercially important orchids in Thailand. Novel microsatellite markers were developed from Mokara, the intergeneric hybrid from 3 genera Vanda, Ascocentrum, and Arachnis by using enriched method.

Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers

M. C. Guidugli, Ferreira-Ramos, R., de Sousa, A. C. B., Cidade, F. W., Marconi, T. G., Mestriner, M. A., Groppo, M., and Alzate-Marin, A. L., “Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers”, vol. 11, pp. 10-16, 2012.

Metrodorea nigra (Rutaceae) is an endemic Brazilian tree of great ecological importance, frequently found in the submontane regions of ombrophilous dense and semideciduous forests. This tree is useful for reforesting degraded areas and the wood can be employed in construction. We developed 12 microsatellite markers from a genomic library enriched for GA/CA repeats, for this species. Polymorphisms were assessed in 40 trees of a highly fragmented population found in Cravinhos, State of São Paulo, in southeastern Brazil.

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Z. - W. Chen, Lu, R. - J., Zou, L., Du, Z. - Z., Gao, R. - H., He, T., and Huang, J. - H., “Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China”, vol. 11, pp. 644-650, 2012.

We analyzed the genetic diversity of 115 barley germplasms, including 112 landraces and three new barley cultivars grown in the Shanghai region, using a set of 11 SSR markers. Sixty-six alleles were observed at the 11 SSR loci, ranged from three to ten, with a mean of six alleles per locus. The polymorphism information content ranged from 0.568 to 0.853, with a mean of 0.732, indicating considerable genetic variation in barley in the Shanghai area. Clustering analysis indicated that these barley accessions could be divided into two categories (A and B).

Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress

M. R. Naroui Rad, M. Kadir, A., Rafii, M. Y., Jaafar, H. Z. E., and Naghavi, M. R., “Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress”, vol. 11, pp. 3882-3888, 2012.

This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under drought stress conditions exhibited continuous variation, indicating the minor gene effects on the trait. Single-marker analysis (SMA) was carried out to detect the main QTL association with drought tolerance.

Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers

G. Q. Wei, Yu, L., Liu, C. L., Zhu, B. J., and Ding, H. J., “Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers”, vol. 12, pp. 2351-2359, 2013.

In the silkworm, Bombyx mori, normal markings are mainly controlled by the +P gene, which is located on the second chromosome. Due to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the +P gene based on an SSR linkage map using silkworm strains P50 and H9, which are normal marking and sex-limited marking, respectively. The +P gene was found to be linked to 3 SSR markers.

Molecular characterization of high performance inbred lines of Brazilian common beans

P. C. B. Cardoso, Veiga, M. M., de Menezes, I. P. P., Valdisser, P. A. M. R., Borba, T. C. O., Melo, L. C., Del Peloso, M. J., Brondani, C., and Vianello, R. P., “Molecular characterization of high performance inbred lines of Brazilian common beans”, vol. 12, pp. 5467-5484, 2013.

The identification of germplasm genetic variability in breeding programs of the common bean (Phaseolus vulgaris) is essential for determining the potential of each combination of parent plants to obtain superior genotypes. The present study aimed to estimate the extent of genetic diversity in 172 lines and cultivars of the common bean by integrating five tests of value for cultivation and use (VCU) that were conducted over the last eight years by the breeding program of Embrapa Arroz e Feijão in Brazil.

Genetic diversity of turmeric germplasm (Curcuma longa; Zingiberaceae) identified by microsatellite markers

M. S. Sigrist, Pinheiro, J. B., Filho, J. A. Azevedo, and Zucchi, M. I., “Genetic diversity of turmeric germplasm (Curcuma longa; Zingiberaceae) identified by microsatellite markers”, vol. 10, pp. 419-428, 2011.

Turmeric (Curcuma longa) is a triploid, vegetatively propagated crop introduced early during the colonization of Brazil. Turmeric rhizomes are ground into a powder used as a natural dye in the food industry, although recent research suggests a greater potential for the development of drugs and cosmetics. In Brazil, little is known about the genetic variability available for crop improvement.

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