Subcellular localization

Cloning and analysis of the soybean MEKK gene

A. - H. Sha, Ba, H. - P., Shan, Z. - H., Chen, H. - F., Chen, S. - L., Qiu, D. - Z., Zhou, X. - A., and Chen, Y. - H., “Cloning and analysis of the soybean MEKK gene”, vol. 14, pp. 3625-3632, 2015.

In this paper, homologous cloning methods were used to clone the soybean GmMEKK gene, which possesses a high degree of similarity to Arabidopsis thaliana AtMEKK1. AtMEKK1 is formed by 595 amino acids, and its secondary structure is formed by 38 irregular curls, 24 α helix, 14 β, with S-TKc domain, transmembrane domain and does not have membrane spanning domain and signal peptide.

Cloning and sequence analysis of the safflower betaine aldehyde dehydrogenase gene

Y. B. Wang, Guan, L. L., Xu, Y. W., Shen, H., and Wu, W., “Cloning and sequence analysis of the safflower betaine aldehyde dehydrogenase gene”, vol. 13, pp. 344-353, 2014.

In response to salinity or drought stress, many plants accumulate glycine betaine, which is a regulator of osmosis. In plants, the last step in betaine synthesis is catalyzed by betaine aldehyde dehydrogenase (BADH), a nuclear-encoded chloroplastic enzyme. Based on the conserved oligo amino acid residues of the published BADH genes from other higher plant species, a cDNA sequence, designated CtBADH, was isolated from safflower (Carthamus tinctorius L.) using a polymerase chain reaction approach.

Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear

J. Tang, Lin, J., Zhang, B. L., Wang, Z. H., Li, X. G., and Chang, Y. H., “Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear”, vol. 13, pp. 8932-8945, 2014.

A 1794-bp cDNA fragment was amplified from mRNA isolated from pear (Pyrus pyrifolia NaKai. Cuiguan) leaves by using primers based on the sequences generated during the analysis of the pear transcriptome. The 597-amino acid sequence encoded by the cDNA was compared with the sequences in GenBank, and it was found to be similar to that of members of the sucrose-proton co-transporter family. The hydrophobic protein, which was predicted to have 11 transmembrane domains, was designated as PpSUT2.

Cloning and sequence analysis of the Blumea balsamifera DC farnesyl diphosphate synthase gene

Y. X. Pang, Guan, L. L., Wu, L. F., Chen, Z. X., Wang, K., Xie, X. L., Yu, F. L., Chen, X. L., Zhang, Y. B., and Jiang, Q., “Cloning and sequence analysis of the Blumea balsamifera DC farnesyl diphosphate synthase gene”, vol. 13, pp. 9874-9882, 2014.

Blumea balsamifera DC is a member of the Compositae family and is frequently used as traditional Chinese medicine. Blumea balsamifera is rich in monoterpenes, which possess a variety of pharmacological activities, such as antioxidant, anti-bacteria, and anti-viral activities. Farnesyl diphosphate synthase (FPS) is a key enzyme in the biosynthetic pathway of terpenes, playing an important regulatory role in plant growth, such as resistance and secondary metabolism.

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