Suppression subtractive hybridization

Identification and characterization of differentially expressed genes during incompatible interaction between the foliar rust Melampsora larici-populina and poplar

Z. J. Chen, Cao, Z. M., and Yu, Z. D., “Identification and characterization of differentially expressed genes during incompatible interaction between the foliar rust Melampsora larici-populina and poplar”, vol. 13, pp. 2082-2093, 2014.

Poplars are extensively cultivated worldwide, and their susceptibility to the foliar rust fungus leads to considerable damages in plantations. To better understand the molecular basis of poplar responses to the foliar rust, suppression subtractive hybridization was used to identify the potential important or novel genes involved in the Populus szechuanica infection by Melampsora larici-populina. A total of 515 expressed sequence tags (ESTs) with high quality were obtained and clustered into 66 contigs and 75 singletons to give a set of 141 uniESTs.

Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9

X. Sun, Xu, N. J., Jiang, L. Z., Hu, W., Fan, M. H., and Liu, Z. M., “Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9”, vol. 13, pp. 8411-8420, 2014.

A strain of the microalga Chlorella pyrenoidosa F-9 in our laboratory showed special characteristics when transferred from autotrophic to heterotrophic culture. In order to elucidate the possible metabolic mechanism, the gene expression profiles of the autonomous organelles in the green alga C. pyrenoidosa under autotrophic and heterotrophic cultivation were compared by suppression subtractive hybridization technology. Two subtracted libraries of autotrophic and heterotrophic C.

Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus

L. Zhu, Li, C. H., Su, X. R., Guo, C. Y., Wang, Z., Jin, C. H., Li, Y., and Li, T. W., “Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus”, vol. 12, pp. 3028-3037, 2013.

Rapid and efficient growth is a major consideration and challenge for global mariculture. The differential growth rate of the sea cucumber, Apostichopus japonicus, has significantly hampered the total production of the industry. In the present study, forward and reverse suppression subtractive hybridization libraries were constructed and sequenced from a fast-growth group and a slow-growth group of the sea cucumber. A total of 142 differentially expressed sequence tags (ESTs) with insertions longer than 150 bp were identified and further analyzed.

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