TaqMan fluorogenic probe

Development of FQ-PCR method to determine the level of ADD1 expression in fatty and lean pigs

J. X. Cui, Chen, W., and Zeng, Y. Q., Development of FQ-PCR method to determine the level of ADD1 expression in fatty and lean pigs, vol. 14, pp. 13924-13931, 2015.

To determine how adipocyte determination and differentiation factor 1 (ADD1), a gene involved in the determination of pork quality, is regulated in Laiwu and Large pigs, we used TaqMan fluorescence quantitative real-time polymerase chain reaction (FQ-PCR) to detect differential expression in the longissimus muscle of Laiwu (fatty) and Large White (lean) pigs. In this study, the ADD1 and GAPDH cDNA sequences were cloned using a T-A cloning assay, and the clone sequences were consistent with those deposited in GenBank.

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