Target-capture sequencing

Single nucleotide polymorphism screening, molecular characterization, and evolutionary aspects of chicken Piwi genes

H. Z. Wang, Ma, T., Chang, G. B., Wan, F., Liu, X. P., Lu, L., Xu, L., Chen, J., and Chen, G. H., Single nucleotide polymorphism screening, molecular characterization, and evolutionary aspects of chicken Piwi genes, vol. 14, pp. 14802-14810, 2015.

The P-element-induced wimpy testis (Piwi) gene is involved in germline stem cell self-renewal, meiosis, RNA silencing, and transcriptional regulation. Piwi genes are relatively well conserved in many species, but their function in poultry species is unclear. In this study, Piwi genes were sequenced using a target-sequence capture assay in quail and 28 breeds of chicken. Single nucleotide polymorphisms (SNPs) and evolutionary aspects of these chicken breeds were then analyzed.

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