TB16.3 protein

Expression and diagnostic value of proteins in Mycobacterium tuberculosis

Z. Y. Zhu, Liu, Y., Wang, H. B., Xiao, J. Z., Qiu, Y. F., Yan, L., Chen, D., Liu, A. G., and Yang, X., Expression and diagnostic value of proteins in Mycobacterium tuberculosis, vol. 13, pp. 7780-7790, 2014.

We constructed a prokaryotic expression vector expressing the Mycobacterium tuberculosis protein TB16.3, as well as 3 other proteins, including TB15.3, CFP-10, and Rv2626C, which were purified and analyzed for their effectiveness as detection antibodies. The TB16.3 genes of M. tuberculosis H37Rv genomic DNA were amplified by polymerase chain reaction, inserted into the expression vector pET-30a, and expressed in Escherichia coli. An enzyme-linked immunosorbent assay was used to detect the 4 M. tuberculosis antibodies. Engineered E.

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