Tegillarca granosa

Development of novel polymorphic microsatellite markers for the blood clam Tegillarca granosa by pyrosequencing

Y. H. Dong, Yao, H. H., Shi, S. F., Bao, Y. B., and Lin, Z. H., Development of novel polymorphic microsatellite markers for the blood clam Tegillarca granosa by pyrosequencing, vol. 14, pp. 8977-8987, 2015.

Large amounts of expressed sequence tags (ESTs) generated using next-generation sequencing technologies provide a cost-effective and valuable genomic resource for the development of microsatellite markers. In this study, we isolated 115 novel polymorphic microsatellite markers for the blood clam Tegillarca granosa from ESTs in 454 sequencing data. All the loci were characterized in 30 individual clams from a natural population in Xiangshan (Zhejiang Province, China). The number of alleles per locus varied from 2 to 10, with an average of 3.78.

Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers

Y. J. Wang, Zeng, Q. G., and Xu, L. N., Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers, vol. 12, pp. 892-900, 2013.

The blood clam, Tegillarca granosa, is widely cultivated in China. We isolated 6 microsatellite loci from T. granosa and used them to investigate genetic diversity and population structure of 5 widely distributed populations of blood clam collected from eastern and southeastern China. The allele number per locus varied from 4 to 9, and the polymorphism information content value was 0.301 to 0.830.

Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides

Y. B. Bao, Li, L., Ye, M. X., Dong, Y. H., Jin, W. X., and Lin, Z. H., Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides, vol. 12, pp. 1143-1154, 2013.

The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase (GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa (Tg-GS) from hemocytes by 3'- and 5'-rapid amplification of cDNA ends (RACE)-PCR. The full-length cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids.

Structure and immune expression analysis of hemoglobin genes from the blood clam Tegillarca granosa

Y. B. Bao, Wang, Q., Guo, X. M., and Lin, Z. H., Structure and immune expression analysis of hemoglobin genes from the blood clam Tegillarca granosa, vol. 12, pp. 3110-3123, 2013.

Hemoglobin (Hb) is the major protein component of erythrocytes in animals with red blood, although it can serve additional functions beyond the transport of oxygen. The blood clam (Tegillarca granosa) is one of the few mollusks that has Hb, although the structure and function of molluskan Hbs remain unclear. We characterized two unique and highly compartmentalized blood clam hemoglobin genes, Tg-HbIIA and Tg-HbIIB, at the molecular level.

Phylogenetic study and barcoding of the blood cockle, Tegillarca granosa, found on the west coast of peninsular Malaysia using the COI gene

S. Y. Chee, Devakie, M. N., and Azizah, M. N. Siti, Phylogenetic study and barcoding of the blood cockle, Tegillarca granosa, found on the west coast of peninsular Malaysia using the COI gene, vol. 10, pp. 1237-1244, 2011.

Blood cockles are among the most economically important brackish water invertebrates found in Malaysia. However, our knowledge of blood cockle phylogeny and systematics is rudimentary, especially for the species Tegillarca granosa. It is unclear, for instance, whether the cockles occurring on the west coast of peninsular Malaysia constitute a single species, or multiple, phylogenetically distinct species. We performed the first DNA molecular phylogenetic analysis of T.

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