Testis

Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes

H. Qiao, Xiong, Y. W., Jiang, S. F., Fu, H. T., Sun, S. M., Jin, S. B., Gong, Y. S., and Zhang, W. Y., Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes, vol. 14, pp. 2041-2054, 2015.

This study utilized high-throughput RNA sequencing technology to identify reproduction- and development-related genes of Macrobrachium nipponense by analyzing gene expression profiles of testis and ovary. More than 20 million 1 x 51-bp reads were obtained by Illumina sequencing, generating more than 7.7 and 11.7 million clean reads in the testis and ovary library, respectively. As a result, 10,018 unitags were supposed to be differentially expressed genes (DEGs) between ovary and testis.

Antibody preparation and identification of the Cashmere goat c-kit protein in the testes

S. C. L. Wu, Luo, F. H., Kong, Q. F., and Wu, Y. J., Antibody preparation and identification of the Cashmere goat c-kit protein in the testes, vol. 13, pp. 4856-4866, 2014.

The c-kit protein plays a major role in the regulation of germ cell development. Its expression and distribution in rodent testes have been widely reported. However, research regarding c-kit expression in domestic animals is scarce, and the expression pattern and distribution of c-kit in germ cells have not been clearly defined. In this study, a specific antigenic region for goat c-kit was designed, and a c-kit polyclonal antibody was prepared. This antibody was then applied in a study evaluating c-kit expression in Cashmere goat tissues.

Novel splice variants of the bovine PCK1 gene

Z. B. Zhang, Zhang, W., Li, R. L., Li, J. B., Zhong, J. F., Zhao, Z. S., and Huang, J. M., Novel splice variants of the bovine PCK1 gene, vol. 12, pp. 4028-4035, 2013.

Phosphoenolpyruvate carboxykinase 1 (PCK1), also named PEPCK-C, is a multiple-function gene that is involved in gluconeogenesis, glyceroneogenesis, reproduction, female fertility, and development of obesity and diabetes. How its many functions are regulated was largely unknown. Therefore, we investigated mRNA expression and possible splice variants of PCK1 by screening cDNA in nine tissues from Holstein bulls and cows.

Expression of acid phosphatase in the seminiferous epithelium of vertebrates

R. L. Peruquetti, Taboga, S. R., and Azeredo-Oliveira, M. T. V., Expression of acid phosphatase in the seminiferous epithelium of vertebrates, vol. 9, pp. 620-628, 2010.

Acid phosphatases (AcPs) are known to provide phosphate to tissues that have high energy requirements, especially during development, growth and maturation. During spermatogenesis AcP activity is manifested in heterophagous lysosomes of Sertoli cells. This phagocytic function appears to be hormone-independent.

A novel human G protein-coupled receptor is over-expressed in prostate cancer

R. B. Parmigiani, Magalhães, G. S., Galante, P. A. F., Manzini, C. V. B., Camargo, A. A., and Malnic, B., A novel human G protein-coupled receptor is over-expressed in prostate cancer, vol. 3, pp. 521-531, 2004.

G protein-coupled receptors (GPCRs) are involved in a large variety of physiological functions. The number of known members that belong to this large family of receptors has been rapidly increasing. Now, with the availability of the human genome sequence databases, further family members are being identified. We describe the identification of a novel GPCR that shows no significant amino acid identity to any one of the known members of the GPCR superfamily.

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