Tissue-specific expression

Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells

H. - Y. Liu, Yang, M. - M., Cui, Y. - H., Ma, T., Liu, X. - Q., Bai, L., Xiao, W. - P., Zhao, H. - B., Peng, J. - Y., Hu, X. - B., and Cao, B. - Y., Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells, vol. 12, pp. 127-135, 2013.

Ovarian-specific promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary DNA library of rat that has been thought to be specifically expressed in ovary. To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding for enhanced green fluorescent protein (EGFP) was used as a reporter to examine the activity of OSP-1 in GCs. EGFP was successfully expressed in dairy goat GCs transfected with pOSP-1-EGFP.

Chicken skeletal muscle-associated macroarray for gene discovery

E. C. Jorge, Melo, C. M. R., Rosário, M. F., Rossi, J. R. S., Ledur, M. C., Moura, A. S. A. M. T., and Coutinho, L. L., Chicken skeletal muscle-associated macroarray for gene discovery, vol. 9, pp. 188-207, 2010.

Macro- and microarrays are well-established technologies to determine gene functions through repeated measurements of transcript abundance. We constructed a chicken skeletal muscle-associated array based on a muscle-specific EST database, which was used to generate a tissue expression dataset of ~4500 chicken genes across 5 adult tissues (skeletal muscle, heart, liver, brain, and skin). Only a small number of ESTs were sufficiently well characterized by BLAST searches to determine their probable cellular functions.

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