Tobacco

Effect of dual Bt-expression transformation vectors on transgene expression in tobacco

L. N. Xu, Dong, Y., Zhang, J., Wang, R. X., Liu, H. M., Yang, Q., Yang, M. S., Xu, L. N., Dong, Y., Zhang, J., Wang, R. X., Liu, H. M., Yang, Q., and Yang, M. S., Effect of dual Bt-expression transformation vectors on transgene expression in tobacco, vol. 15, p. -, 2016.

This study aimed to determine the influence of vector structure on dual Bt gene expression and establish an efficient expression vector using Cry1Ac and Cry3A genes. Four vectors (N4, N5, N10, and S23) were developed and used for genetic transformation of tobacco to obtain insect-resistant transgenic lines. The vectors were constructed using the MAR structure, applying different promoter and enhancer sequences, and changing the transgene open-reading frame sequence.

De novo transcriptome analysis of tobacco seedlings and identification of the early response gene network under low-potassium stress

L. Q. Li, Li, J., Chen, Y., Lu, Y. F., Lu, L. M., Li, L. Q., Li, J., Chen, Y., Lu, Y. F., and Lu, L. M., De novo transcriptome analysis of tobacco seedlings and identification of the early response gene network under low-potassium stress, vol. 15, p. -, 2016.

Tobacco is an economically important crop, and its potassium content can greatly affect the quality of tobacco leaves. However, the molecular mechanism involved in potassium starvation in tobacco has not been elucidated to date. In this study, Illumina (Solexa) sequencing technology was used to analyze the transcriptome of tobacco seedlings under low-potassium stress for 6, 12, and 24 h. After analysis, 107,824 assembled unigenes were categorized into 57 GO functional groups, and 31,379 unigenes (29.08%) were clustered into 25 COG categories.

Identification of low potassium stress-responsive proteins in tobacco (Nicotiana tabacum) seedling roots using an iTRAQ-based analysis

X. L. Ren, Li, L. Q., Xu, L., Guo, Y. S., Lu, L. M., Ren, X. L., Li, L. Q., Xu, L., Guo, Y. S., and Lu, L. M., Identification of low potassium stress-responsive proteins in tobacco (Nicotiana tabacum) seedling roots using an iTRAQ-based analysis, vol. 15, p. -, 2016.

Potassium is one of the three main mineral nutrients, and is vital for leaf growth and the quality of tobacco (Nicotiana tabacum) plants. In recent years, the isobaric tags for relative and absolute quantitation (iTRAQ) method has been one of the most popular techniques for quantitative proteomic analysis. In this study, we used iTRAQ to compare protein abundances in the roots of control and low potassium-treated tobacco seedlings, and found that 108 proteins were differentially expressed between the two treatments.

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