Tocopherol cyclase

Molecular cloning and characterization of a tocopherol cyclase gene from Lactuca sativa (Asteraceae)

Y. L. Tang, Ren, W. W., Zhang, L., and Tang, K. X., Molecular cloning and characterization of a tocopherol cyclase gene from Lactuca sativa (Asteraceae), vol. 10, pp. 693-702, 2011.

Tocopherol cyclase is a rate-limiting enzyme involved in tocopherol biosynthesis. The full-length cDNA encoding tocopherol cyclase (designated as LsTC) was cloned from lettuce (Lactuca sativa) for the first time by rapid amplification of cDNA ends (RACE) and characterized by means of quantitative RT-PCR. The full-length cDNA of LsTC was 1675 bp, with an open reading frame of 1521 bp, encoding a tocopherol cyclase protein of 506 amino acids, with a calculated molecular mass of 56.76 kD and an isoelectric point of 6.49.

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