Using the RNA sequencing, transcriptomes of growth-inhibited rice seedlings induced by low-energy N+-beam implantation were analyzed. The results showed that rice genes related to binding, electron carrier activity, transcription factor, auxin signal transduction, photosynthesis, and oxidoreductase activity were involved in the response to ion-beam implantation. Moreover, low-energy N+-beam implantation can induce the reactivation of the repressed rice transposable elements. These data are a useful resource for plant ion-beam implantation research.
Transcriptomic research based on RNA sequencing (RNA-seq) technology is innovative and will provide new opportunities and biological perspectives for the use of ion-beam implantation in plants. Using RNA-seq, transcriptomes of whole rice seedlings generated from seeds implanted with a low-energy N+ beam were analyzed 96 h after planting. We identified 544 transcripts that were differentially expressed genes (DEGs) in the non-growth-inhibited sample implanted by an N+ beam, including 262 upregulated transcripts and 282 downregulated transcripts.