Transcriptome

Development, characterization, and annotation of potential simple sequence repeats by transcriptome sequencing in pears (Pyrus pyrifolia Nakai)

H. Zhou, Cai, B. H., Lu, Z. Q., Gao, Z. H., Qiao, Y. S., Zhou, H., Cai, B. H., Lu, Z. Q., Gao, Z. H., and Qiao, Y. S., Development, characterization, and annotation of potential simple sequence repeats by transcriptome sequencing in pears (Pyrus pyrifolia Nakai), vol. 15, p. -, 2016.

Simple sequence repeats (SSRs), one of the most powerful molecular markers, can be used for DNA fingerprinting, variety identification, genetic mapping, and marker-assisted selection. Using the pear’s (Pyrus pyrifolia Nakai) 75,764 unigenes (55,676,271 bp) obtained by deep transcriptome sequencing, a total of 10,622 novel SSRs were identified in 9154 unigenes, accounting for 14.02% of all unigenes. The average length and distribution of these SSRs was about 16 bp and 5.24 kb, respectively.

Bioinformatic analyses of GRAS genes in Betula kirghisorum based on transcriptome data

C. J. Yang, Li, G. Y., Cui, Y. L., Yang, C. J., Li, G. Y., and Cui, Y. L., Bioinformatic analyses of GRAS genes in Betula kirghisorum based on transcriptome data, vol. 15, p. -, 2016.

The transcriptomes of salt-stressed and unstressed Betula kirghisorum plants were analyzed using high throughput sequencing technology. A total of 52,239,804 and 51,772,998 clean reads were obtained from the two libraries, respectively, and de novo assembled into 60,545 all-unigenes. A total of 39,997 unigenes were annotated using public databases. Overall, 7206 genes were differentially expressed in unigenes and were involved in 127 pathways. Thirteen transcription factor families were identified in B.

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