Transfection

Behavior of calf Sertoli cells and fibroblast cells transfected with the human HNP-1 gene

N. Yu, Khalid, A., Shi, Y., Liu, X., Lin, X., Li, Y. L., and Zhang, G. X., Behavior of calf Sertoli cells and fibroblast cells transfected with the human HNP-1 gene, vol. 13, pp. 9656-9664, 2014.

Human neutrophil peptide-1 (HNP-1) is an important defense molecule in neutrophils and Sertoli cells and plays an important role in the blood-testis barrier. In this study, we investigated the behavior of Sertoli cells transfected with the HNP-1 gene and compared the ability of Sertoli cells and fibroblast cells to resist transfection. Total RNA was isolated from human blood.

Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells

L. T. Iguma, Iguma, L. T., Melo, E. O., Franco, M. M., Pivato, I., Vianna, G. R., Sousa, R. V., Dode, M. A. N., Aragão, F. J. L., Rech, E. L., Rech, E. L., Rumpf, R., and Lisauskas, S. F. C., Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells, vol. 4, pp. 55-66, 2005.

An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo developmental capacities of NT using fibroblast cells isolated from a 14-month-old cloned Simmental heifer (FCE) vs the same line transfected with a plasmid containing neomycin-resistant genes (TFCE).

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