Transferability

Sequence divergence of microsatellites for phylogeographic assessment of Moroccan Medicago species

N. Zitouna, Marghali, S., Gharbi, M., Haddioui, A., and Trifi-Farah, N., Sequence divergence of microsatellites for phylogeographic assessment of Moroccan Medicago species, vol. 13, pp. 1548-1562, 2014.

Six Medicago species were investigated to characterize and valorize plant genetic resources of pastoral interest in Morocco. Samples were obtained from the core collection of the South Australian Research and Development Institute (SARDI). The transferability of single sequence repeat markers of Medicago truncatula was successful with 97.6% efficiency across the five species.

Isolation and characterization of microsatellites for the yam Dioscorea cayenensis (Dioscoreaceae) and cross-amplification in D. rotundata

L. R. G. Silva, Bajay, M. M., Monteiro, M., Mezette, T. F., Nascimento, W. F., Zucchi, M. I., Pinheiro, J. B., and Veasey, E. A., Isolation and characterization of microsatellites for the yam Dioscorea cayenensis (Dioscoreaceae) and cross-amplification in D. rotundata, vol. 13, pp. 2766-2771, 2014.

Dioscorea cayenensis and Dioscorea rotundata are among the most important yam species for the humid and sub-humid tropics. We isolated nine polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. The nine primer pairs were validated in 22 D. cayenensis accessions, and were tested for transferability in 26 D. rotundata accessions. The number of bands ranged from 2 to 4, with a mean of 3.11. D. cayenensis gave primer polymorphism information content values ranging from 0.37 to 0.62, while for D.

Isolation and characterization of novel microsatellite markers from Siamese fighting fish (Betta splendens, Osphronemidae, Anabantoidei) and their transferability to related species, B. smaragdina and B. imbellis

V. Chailertrit, Swatdipong, A., Peyachoknagul, S., Salaenoi, J., and Srikulnath, K., Isolation and characterization of novel microsatellite markers from Siamese fighting fish (Betta splendens, Osphronemidae, Anabantoidei) and their transferability to related species, B. smaragdina and B. imbellis, vol. 13, pp. 7157-7162, 2014.

Ten novel microsatellite markers were developed and characterized from Siamese fighting fish (Betta splendens). Nine of ten markers were polymorphic, exhibiting an allelic number (NA) from 2 to 6 alleles per locus. The effective number of alleles (NE) ranged from 1.60 to 3.08 (average of 2.30). The observed (HO) and expected (HE) heterozygosities ranged from 0.13 to 0.67 (average of 0.39) and 0.29 to 0.63 (average of 0.50), respectively.

Cross-species transferability of microsatellite markers in the genus Lippia

C. P. Santos, Rocha, D. S., Bajay, M. M., Santos, F. R. C., Campos, J. B., Pinheiro, J. B., Zucchi, M. I., Silva-Mann, R., Arrigoni-Blank, M. F., and Blank, A. F., Cross-species transferability of microsatellite markers in the genus Lippia, vol. 13, pp. 9846-9850, 2014.

The cross-species transferability of 20 microsatellite markers was tested in the genus Lippia. Eleven markers were polymorphic after screening 19 accessions of Lippia sidoides and Lippia gracilis maintained in the Active Germplasm Bank (AGB) from Universidade Federal de Sergipe. Additionally, 40 accessions of Lippia spp were collected in Sergipe to increase the germplasm bank. A total of 23, 22, and 36 alleles were identified, with an average of 2.3, 2.2, and 3.27 alleles per locus, respectively, for each group.

Thirty-four Musa (Musaceae) expressed sequence tag-derived microsatellite markers transferred to Musella lasiocarpa

W. J. Li, Ma, H., Li, Z. H., Wan, Y. M., Liu, X. X., and Zhou, C. L., Thirty-four Musa (Musaceae) expressed sequence tag-derived microsatellite markers transferred to Musella lasiocarpa, vol. 11, pp. 2094-2098, 2012.

We assembled 31,308 publicly available Musa EST sequences into 21,129 unigenes; 4944 of them contained 5416 SSR motifs. In all, 238 unigenes flanking SSRs were randomly selected for primer design and then tested for amplification in Musella lasiocarpa. Seventy-eight primer pairs were found to be transferable to this species, and 49 displayed polymorphism. A set of 34 polymorphic SSR markers was analyzed in 24 individuals from four wild M. lasiocarpa populations. The mean number of alleles per locus was 3.0, ranging from 2 to 7.

Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species

X. Y. Du, Hu, Q. N., Zhang, Q. L., Wang, Y. B., and Luo, Z. R., Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species, vol. 12, pp. 1781-1795, 2013.

Retrotransposon-based molecular markers are powerful molecular tools. However, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. Although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-consuming and costly. Therefore, research into the transferability of retrotransposon primers developed from one plant species onto others would be of great value.

Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species

Q. - Q. Yan, Sun, X. - Q., Guo, J. - L., Hang, Y. - Y., and Li, M. - M., Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species, vol. 12, pp. 3788-3792, 2013.

Dioscorea zingiberensis C.H. Wright (Dioscoreaceae) is an endemic species in central and southwestern China. In order to study the genetic diversity and population structure of this species, 19 novel polymorphic microsatellite loci were developed using a dual-suppression PCR technique. The number of alleles per locus ranged from 3 to 21, with an average of 9.53. All the markers showed high transferability in cross-species amplification in other species of sect. Stenophora.

Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici

A. M. Olivatti, Boni, T. A., Silva-Júnior, N. J., Resende, L. V., Gouveia, F. O., and Telles, M. P. C., Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici, vol. 10. pp. 1403-1408, 2011.

Leporinus friderici, native to the Amazon Basin and popularly known as “piau-três-pintas”, has great ecological and economic importance; it is widely fished and consumed throughout much of tropical South America. Knowledge of the genetic diversity of this native species is important to support management and conservation programs. We evaluated microsatellite loci amplification, using heterologous primers, in 31 individuals of L. friderici.

Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona

M. V. C. Viana, Miranda, E. A., de Francisco, A. K., Carvalho, C. A. L., and Waldschmidt, A. M., Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona, vol. 10, pp. 3942-3947, 2011.

Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intracolonial genetic analyses. The genus Melipona has the largest number of species among bee genera, about 70, occurring throughout the Neotropical region. However, only five species of the genus Melipona have specific microsatellite markers.

Transferability of microsatellite loci from Cervidae species to the endangered Brazilian marsh deer, Blastocerus dichotomus

K. C. E. Leite, Collevatti, R. G., Menegasso, T. R., Tomas, W. M., and Duarte, J. M. B., Transferability of microsatellite loci from Cervidae species to the endangered Brazilian marsh deer, Blastocerus dichotomus, vol. 6, pp. 325-330, 2007.

Blastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroelectric power station construction and agricultural land expansion. In the present study, we tested 38 microsatellite loci from four Cervidae species: Odocoileus virginianus (7), Rangifer tarandus (17), Capreolus capreolus (7), and Mazama bororo (7).

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