Breeding of transgenic cattle for human coagulation factor IX by a combination of lentiviral system and cloning
“Breeding of transgenic cattle for human coagulation factor IX by a combination of lentiviral system and cloning”, vol. 12, pp. 3675-3688, 2013.
, Recombinant coagulation factor IX must be produced in mammalian cells because FIX synthesis involves translational modifications. Human cell culture-based expression of human coagulation factor IX (hFIX) is expensive, and large-scale production capacity is limited. Transgenic animals may greatly increase the yield of therapeutic proteins and reduce costs. In this study, we used a lentiviral system to obtain transgenic cells and somatic cell nuclear transfer (SCNT) to produce transgenic animals.