Transgenic cloned cattle

Breeding of transgenic cattle for human coagulation factor IX by a combination of lentiviral system and cloning

P. S. Monzani, Sangalli, J. R., de Bem, T. H. C., Bressan, F. F., Fantinato-Neto, P., Pimentel, J. R. V., Birgel-Junior, E. H., Fontes, A. M., Covas, D. T., and Meirelles, F. V., Breeding of transgenic cattle for human coagulation factor IX by a combination of lentiviral system and cloning, vol. 12, pp. 3675-3688, 2013.

Recombinant coagulation factor IX must be produced in mammalian cells because FIX synthesis involves translational modifications. Human cell culture-based expression of human coagulation factor IX (hFIX) is expensive, and large-scale production capacity is limited. Transgenic animals may greatly increase the yield of therapeutic proteins and reduce costs. In this study, we used a lentiviral system to obtain transgenic cells and somatic cell nuclear transfer (SCNT) to produce transgenic animals.

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