In the present study, we isolated an Arabidopsis promoter, UGT71C5, and analyzed its role in the regulation of the light response mechanism. We constructed a fusion vector pBI121-pU-GUS by integrating the UGT71C5 promoter upstream of the GUS reporter gene in pBI121, and then transferred this vector into Arabidopsis plants. The GUS activity of the transgenic plants was detected using a spectrophotometer under normal growth conditions as well as under light, drought, and ABA stress-treatments.
The kiwifruit (Actinidia chinensis Planch.) is an economically and nutritionally important fruit crop that has a remarkably high vitamin C content and is popular throughout the world. However, kiwifruit plants are vulnerable to attack from pests, and effective pest control is urgently required. Transgenic kiwifruit plants containing the synthetic chimeric gene SbtCry1Ac that encodes the insecticidal protein btCrylAc were obtained through an Agrobacterium-mediated transformation of kiwifruit leaf discs.