Trichoderma

A rapid and inexpensive method for isolation of total DNA from Trichoderma spp (Hypocreaceae)

J. C. Vazquez-Angulo, Mendez-Trujillo, V., González-Mendoza, D., Morales-Trejo, A., Grimaldo-Juarez, O., and Cervantes-Díaz, L., A rapid and inexpensive method for isolation of total DNA from Trichoderma spp (Hypocreaceae), vol. 11. pp. 1379-1384, 2012.

Extraction of high-quality genomic DNA for PCR amplification from filamentous fungi is difficult because of the complex cell wall and the high concentrations of polysaccharides and other secondary metabolites that bind to or co-precipitate with nucleic acids. We developed a modified sodium dodecyl sulfate/phenol protocol, without maceration in liquid nitrogen and without a final ethanol precipitation step. The A260/280 absorbance ratios of isolated DNA were approximately 1.7-1.9, demonstrating that the DNA fraction is pure and can be used for analysis.

M13-microsatellite PCR and rDNA sequence markers for identification of Trichoderma (Hypocreaceae) species in Saudi Arabian soil

K. A. Abd-Elsalam, Almohimeed, I., Moslem, M. A., and Bahkali, A. H., M13-microsatellite PCR and rDNA sequence markers for identification of Trichoderma (Hypocreaceae) species in Saudi Arabian soil, vol. 9, pp. 2016-2024, 2010.

Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed spacer sequences of ribosomal DNA were used to confirm the identity of the two Trichoderma species.

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