Triticum aestivum

Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay

E. Abdelhaliem, Al-Huqail, A. A., Abdelhaliem, E., and Al-Huqail, A. A., Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay, vol. 15, p. -, 2016.

This study was designed to compare the genetic effects of elevated carbon dioxide (CO2) and ozone (O3), alone or in combination, under irrigated and non-irrigated conditions on proteins and DNA of wheat (Triticum aestivum L.). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isozymes, random amplified polymorphic DNA (RAPD), and comet assays were used.

Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat

M. Ejaz, Qidi, Z., Gaisheng, Z., Na, N., Huiyan, Z., and Qunzhu, W., Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat, vol. 14, pp. 8935-8946, 2015.

Amplified fragment length polymorphism markers were used to assess genetic diversity in 10 male sterile wheat crop lines (hetero-cytoplasm with the same nucleus) in relation to a restorer wheat line. These male sterile lines were evaluated using 64 amplified fragment length polymorphism primer combinations, and 13 primers produced polymorphic bands, generating a total 682 fragments. Of the 682 fragments, 113 were polymorphic. The polymorphic information content and marker index values demonstrated the utility of the primer combinations used in the present study.

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