Ubiquitin

Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress

G. P. Moraes, Benitez, L. C., Amaral, M. Ndo, Vighi, I. L., Auler, P. A., da Maia, L. C., Bianchi, V. J., and Braga, E. J. B., Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress, vol. 14, pp. 2384-2398, 2015.

To obtain accurate and reliable results for the expression of genes of interest using quantitative real-time polymerase chain reaction (RT-qPCR) techniques, it is necessary to normalize the data by comparing them to constitutive genes that exhibit uniform expression levels under experimental conditions.

Comparative fusion expression of maize SINAT5 in two different strains of Escherichia coli

A. Gholizadeh, Comparative fusion expression of maize SINAT5 in two different strains of Escherichia coli, vol. 11, pp. 2760-2768, 2012.

SINAT5 is a plant E3 ligase that regulates auxin signaling and root morphogenesis by ubiquitination of the NAC1 protein. Consequently, it may be a putative regulator of aspects of plant development cycles that are controlled by auxin. Efficient production, purification and correctly folded form of this protein are important requirements for functional studies. We produced and quantitatively compared fusion expression of the “maltose binding protein (mbp)-maize sinat5” construct in two different strains of Escherichia coli.

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