Vascular endothelial growth factor 121

Construction of an adenoviral expression vector carrying FLAG and hrGFP-1 genes and its expression in bone marrow mesenchymal stem cells

G. X. Wang, Hu, L., Zhang, Z., and Liu, D. P., Construction of an adenoviral expression vector carrying FLAG and hrGFP-1 genes and its expression in bone marrow mesenchymal stem cells, vol. 13, pp. 1070-1078, 2014.

The aim of this study was to construct an adenoviral expression vector for vascular endothelium growth factor 121 (VEGF121)-FLAG and humanized Renilla reniformis green fluorescent protein (hrGFP-1) genes, and to observe their expressions in bone marrow mesenchymal stem cells. Using pTG19T-VEGF121 as a template, polymerase chain reaction technology was adopted to mutate the VEGF121 gene by removing the stop codon and inserting NotI and XhoI restriction sites both before and after the gene sequences.

Subscribe to Vascular endothelial growth factor 121