Verticillium wilt

Methylation-sensitive amplified polymorphism analysis of Verticillium wilt-stressed cotton (Gossypium)

W. Wang, Zhang, M., Chen, H. D., Cai, X. X., Xu, M. L., Lei, K. Y., Niu, J. H., Deng, L., Liu, J., Ge, Z. J., Yu, S. X., Wang, B. H., Wang, W., Zhang, M., Chen, H. D., Cai, X. X., Xu, M. L., Lei, K. Y., Niu, J. H., Deng, L., Liu, J., Ge, Z. J., Yu, S. X., and Wang, B. H., Methylation-sensitive amplified polymorphism analysis of Verticillium wilt-stressed cotton (Gossypium), vol. 15, p. -, 2016.

In this study, a methylation-sensitive amplification polymorphism analysis system was used to analyze DNA methylation level in three cotton accessions. Two disease-sensitive near-isogenic lines, PD94042 and IL41, and one disease-resistant Gossypium mustelinum accession were exposed to Verticillium wilt, to investigate molecular disease resistance mechanisms in cotton. We observed multiple different DNA methylation types across the three accessions following Verticillium wilt exposure. These included hypomethylation, hypermethylation, and other patterns.

Analysis of upland cotton (Gossypium hirsutum) response to Verticillium dahliae inoculation by transcriptome sequencing

B. X. Shao, Zhao, Y. L., Chen, W., Wang, H. M., Guo, Z. J., Gong, H. Y., Sang, X. H., Cui, Y. L., and Wang, C. H., Analysis of upland cotton (Gossypium hirsutum) response to Verticillium dahliae inoculation by transcriptome sequencing, vol. 14, pp. 13120-13130, 2015.

Verticillium wilt is one of the main diseases in cotton (Gossypium hirsutum), severely reduces yield and fiber quality, and is difficult to be con­trolled effectively. At present, the molecular mechanism that confers resistance to this disease is unclear. Transcriptome sequencing is an important method to detect resistance genes, explore metabolic pathways, and study resistance mechanisms. In this study, the transcriptome of a disease-resistant inbred cot­ton line inoculated with Verticillium dahliae was sequenced.

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