A novel method for the evaluation of virus-induced gene silencing efficiency
“A novel method for the evaluation of virus-induced gene silencing efficiency”, vol. 13, pp. 9443-9452, 2014.
, Virus-induced gene silencing (VIGS) is an important tool for studying gene function. However, a number of factors highly restrict the application of VIGS, such as unstable efficiency and tissue-specific silencing. We developed a novel evaluation method for improving the applicability of VIGS vectors. In this method, 4 indexes were defined and utilized to evaluate VIGS efficiency by silencing the endogenous phytoene desaturase (PDS) gene with a tobacco rattle virus-based VIGS vector.