Western blot

Alteration of Phascolosoma esculenta heat shock protein 90 expression under heavy metal exposure and thermal stress

C. H. Li, Su, X. R., Wang, M. Q., Li, Y. Y., Li, Y., Zhou, J., and Li, T. W., “Alteration of Phascolosoma esculenta heat shock protein 90 expression under heavy metal exposure and thermal stress”, vol. 11, pp. 2641-2651, 2012.

The full-length complementary DNA (cDNA) of heat shock protein 90 was cloned from Phascolosoma esculenta (PeHSP90) using expressed sequence tag and rapid amplification of cDNA end approaches. The cDNA of PeHSP90 was 2521 bp including a 5'-untranslated region of 110 bp, a 3'-untranslated region of 230 bp, and an open reading frame of 2181 bp. All of the characteristic motifs of the HSP90 family were completely conserved in the deduced amino acid of PeHSP90.

Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep

L. H. Wang, Zhang, W., Ji, J. L., Gao, Q. X., Xiao, S. H., and Wang, F., “Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep”, vol. 12, pp. 1490-1500, 2013.

Liver receptor homologue 1 (Lrh-1) is a member of the nuclear receptor belonging to the second subfamily of the nuclear receptor family 5A (NR5A), also named NR5A2, which is important for lipid homeostasis, embryogenesis, and regulation of aromatics. The present study aimed to understand the sequence of ovine Lrh-1 and the expression traits in reproductive organ tissues. Initially, we cloned Lrh-1 from the liver of Hu sheep through degenerate primer of reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends.

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