Western blotting

Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa

J. J. Cui, Tian, Y., Liu, Y., Huang, H. L., and Jin, Y., Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa, vol. 13, pp. 9915-9920, 2014.

The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-thawed spermatozoa, which were smaller than those of the fresh group.

Fast preparation of a polyclonal antibody against chicken protocadherin 1

Y. H. Li, Zhao, X. H., Li, M., Yang, C. Q., Wang, L., and Lin, J. T., Fast preparation of a polyclonal antibody against chicken protocadherin 1, vol. 12, pp. 2156-2166, 2013.

Protocadherins constitute a large family belonging to the cadherin superfamily; they function in various tissues of a wide variety of multicellular organisms. However, their functions and expression modes are still unknown in many of these species and tissues. We developed a fast and low-cost method to produce polyclonal antibody against chicken protocadherin 1 (Pcdh1) that could be used in assays for immunological assessment of protein expression levels of chicken Pcdh1.

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