Western blotting

Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig

Y. Y. Bao, Li, L., Zhang, H., Gao, C. Y., Xiao, C. B., and Li, C. L., Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig, vol. 14, pp. 18863-18871, 2015.

Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits to generate polyclonal antiserum. Anti-pBD2 antibodies were purified by ammonium sulfate precipitation, followed by diethylaminoethyl cellulose ion-exchange chromatography.

Subscribe to Western blotting