Western blotting

Genetic effect of an A/G polymorphism in the HSP70 gene on thermotolerance in chicken

Z. Y. Chen, Zhang, W. W., Gan, J. K., Kong, L. N., Zhang, X. Q., Zhang, D. X., Luo, Q. B., Chen, Z. Y., Zhang, W. W., Gan, J. K., Kong, L. N., Zhang, X. Q., Zhang, D. X., and Luo, Q. B., “Genetic effect of an A/G polymorphism in the HSP70 gene on thermotolerance in chicken”, vol. 15, p. -, 2016.

Studying thermotolerance is important for the prevention of thermostress in chickens. This study aimed to analyze the effect of mutations in the heat shock protein 70 (HSP70) gene on chicken thermotolerance. The C.-69A>G SNP in the 5'-flanking region of the HSP70 gene was genotyped in Lingshan and White Recessive Rock (WRR) chickens.

Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig

Y. Y. Bao, Li, L., Zhang, H., Gao, C. Y., Xiao, C. B., and Li, C. L., “Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig”, vol. 14, pp. 18863-18871, 2015.

Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits to generate polyclonal antiserum. Anti-pBD2 antibodies were purified by ammonium sulfate precipitation, followed by diethylaminoethyl cellulose ion-exchange chromatography.

Expression of OPG, RANKL, and RUNX2 in rabbit periodontium under orthodontic force

B. Li, Zhang, Y. H., Wang, L. X., Li, X., and Zhang, X. D., “Expression of OPG, RANKL, and RUNX2 in rabbit periodontium under orthodontic force”, vol. 14, pp. 19382-19388, 2015.

This study aims to investigate the expression changes of RANKL, RUNX2, and OPG in rabbit periodontal tissues under orthodontic force and explore its effect on the remodeling of periodontal tissues. A total of 16 specific pathogen-free rabbits were used in this study. The maxillary appliance was worn on the right (experimental) side, and the appliance-free left side was used as the control. Rabbits were sacrificed after 3, 5, 7, and 14 days of treatment.

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