Wheat

Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat

M. J. Wang, Zou, H. D., Lin, Z. S., Wu, Y., Chen, X., and Yuan, Y. P., Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat, vol. 11, pp. 3452-3463, 2012.

We developed EST-PCR markers specific to barley chromosome 2H, for the purpose of effectively tracing alien chromosomes or chromosome parts in the wheat genetic background. The target alien chromosome 2H confers high resistance to pre-harvest sprouting, which is a worldwide natural disaster in wheat. A total of 120 primer pairs were selected by combining the wheat group 2 chromosomes of the EST database and the genome sequences of the new model plant Brachypodium distachyon.

Isolation and in silico functional analysis of MtATP6, a 6-kDa subunit of mitochondrial F1F0-ATP synthase, in response to abiotic stress

A. A. Moghadam, Taghavi, S. M., Niazi, A., Djavaheri, M., and Ebrahimie, E., Isolation and in silico functional analysis of MtATP6, a 6-kDa subunit of mitochondrial F1F0-ATP synthase, in response to abiotic stress, vol. 11, pp. 3547-3567, 2012.

Mitochondrial F1F0-ATP synthase is a key enzymatic complex of energy metabolism that provides ATP for the cell. Subunits of this enzyme over-express under stress conditions. Little is known about the structure and regulatory mechanism of the F0 portion of this enzyme. We isolated the full-length coding sequence of the RMtATP6 gene from rice and wheat, and partial sequences from Aegilops crassa and Triticum monococcum (Poaceae).

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