Witches’ broom disease

Genetic diversity of polysporic isolates of Moniliophthora perniciosa (Tricholomataceae)

L. F. R. Ferreira, Duarte, K. M. R., Gomes, L. H., Carvalho, R. S., Junior, G. A. Leal, Aguiar, M. M., Armas, R. D., and Tavares, F. C. A., Genetic diversity of polysporic isolates of Moniliophthora perniciosa (Tricholomataceae), vol. 11, pp. 2559-2568, 2012.

The causal agent of witches’ broom disease, Moniliophthora perniciosa is a hemibiotrophic and endemic fungus of the Amazon basin and the most important cocoa disease in Brazil. The purpose of this study was to analyze the genetic diversity of polysporic isolates of M. perniciosa to evaluate the adaptation of the pathogen from different Brazilian regions and its association with different hosts. Polysporic isolates obtained previously in potato dextrose agar cultures of M.

Single nucleotide polymorphisms from Theobroma cacao expressed sequence tags associated with witches’ broom disease in cacao

L. S. Lima, Gramacho, K. P., Carels, N., Novais, R., Gaiotto, F. A., Lopes, U. V., Gesteira, A. S., Zaidan, H. A., Cascardo, J. C. M., Pires, J. L., and Micheli, F., Single nucleotide polymorphisms from Theobroma cacao expressed sequence tags associated with witches’ broom disease in cacao, vol. 8, pp. 799-808, 2009.

In order to increase the efficiency of cacao tree resistance to witches’ broom disease, which is caused by Moniliophthora perniciosa (Tricholomataceae), we looked for molecular markers that could help in the selection of resistant cacao genotypes. Among the different markers useful for developing marker-assisted selection, single nucleotide polymorphisms (SNPs) constitute the most common type of sequence difference between alleles and can be easily detected by in silico analysis from expressed sequence tag libraries.

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