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Z. C. Long, Gichira, A. W., Chen, J. M., Wang, Q. F., Liao, K., and A, Development of EST-SSR markers in the relict tree Davidia involucrata (Davidiaceae) using transcriptome sequencing, vol. 15, no. 4, p. -, 2016.
Conflicts of interestThe authors declare no conflict of interestACKNOWLEDGMENTSWe thank Zhi-Yuan Du, Le-Na Li, and Hua Liu for their help in the laboratory. Research supported by grants from the National Natural Science Foundation of China (#31200170 and #31570220). REFERENCESChen JM, Zhao SY, Liao YY, Gichira AW, et al (2015). Chloroplast DNA phylogeographic analysis reveals significant spatial genetic structure of the relictual tree Davidia involucrata (Davidiaceae). Conserv. Genet. 16: 583-593. Chen Z, Xue C, Zhu S, Zho F, et al (2005). GoPipe: streamlined gene ontology annotation for batch anonymous sequences with statistics. Prog. Biochem. Biophys. 32: 187-191. Du YJ, Dai QY, Zhang LY, Qiu YX, et al (2012). Development of microsatellite markers for the dove tree, Davidia involucrata (Nyssaceae), a rare endemic from China. Am. J. Bot. 99: e206-e209. Fu LK and Jin JM (1992). China plant red data book - rare and endangered plants. Vol. 1. Science Press, Beijing. Grabherr MG, Haas BJ, Yassour M, Levin JZ, et al (2011). Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29: 644-652. Kanehisa M, Goto S, Furumichi M, Tanabe M, et al (2010). KEGG for representation and analysis of molecular networks involving diseases and drugs. Nucleic Acids Res. 38: D355-D360. Li XP, Li ZL, He CL, Zhu WY, et al (2012a). Genetic diversity of the endangered Davidia involucrata by AFLP analysis. Acta Hort. Sin. 39: 992-998. Li Z, Wang C, Liu Y, Li J, et al (2012b). Microsatellite primers in the Chinese dove tree, Davidia involucrata (Cornaceae), a relic species of the Tertiary. Am. J. Bot. 99: e78-e80. Luo S, He Y, Ning G, Zhang J, et al (2011). Genetic diversity and genetic structure of different populations of the endangered species Davidia involucrata in China detected by inter-simple sequence repeat analysis. Trees 25: 1063-1071. Ma Q, Du YJ, Chen N, Zhang LY, et al (2015). Phylogeography of Davidia involucrata (Davidiaceae) inferred from cpDNA haplotypes and nSSR data. Syst. Bot. 40: 796-810. Manchester SR, et al (2002). Leaves and fruits of Davidia (Cornales) from the Paleocene of North America. Syst. Bot. 27: 368-382. Ng P, Wei CL, Sung WK, Chiu KP, et al (2005). Gene identification signature (GIS) analysis for transcriptome characterization and genome annotation. Nat. Methods 2: 105-111. Peakall R, Smouse PE, et al (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update. Bioinformatics 28: 2537-2539. Peng YL, Hu YQ, Sun H, et al (2003). Allozyme analysis of Davidia involucrata var. vilmoriniana and its biogeography significance Acta Bot. Yunnanica 25: 55-62. Rice P, Longden I, Bleasby A, et al (2000). EMBOSS: the European molecular biology open software suite. Trends Genet. 16: 276-277. Rozen S, Skaletsky H, et al (2000). Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132: 365-386. Song CW, Bao MZ, et al (2004). Study on genetic diversity of RAPD mark for natural Davidia involucrata population. Sci. Silvae Sin. 40: 75-79. Tang CQ, Ohsawa M, et al (2002). Tertiary relic deciduous forests on a humid subtropical mountain, Mt. Emei, Sichuan, China. Folia Geobot. 37: 93-106. Tao C, Yang Z, Lu R, Liu T, et al (2012). Microsatellite markers for the relictual dove tree, Davidia involucrata (Cornaceae). Am. J. Bot. 99: e108-e110. Wu J, Cai C, Cheng F, Cui H, et al (2014). Characterisation and development of EST-SSR markers in tree peony using transcriptome sequences. Mol. Breed. 34: 1853-1866. You H, Fujiwara K, Liu Y, et al (2014). A preliminary vegetation-ecological study of Davidia involucrata forest. Nat. Sci. 6: 1012-1029. Zhang J, Ma W, Song X, Lin Q, et al (2014). Characterization and development of EST-SSR markers derived from transcriptome of yellow catfish. Molecules 19: 16402-16415.