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2016
B. Zeng, Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., Mu, Q., Zeng, B., Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., Mu, Q., Zeng, B., Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., and Mu, Q., Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi, vol. 15, no. 4, p. -, 2016.
Conflicts of interest The authors declare no conflict of interest. ACKNOWLEDGMENTS Research supported by the Youth Project Foundation of Rongchang Campus, Southwest University, “The germplasm resources of Dactylis glomerata in rust resistance evaluation and development of rust-resistant gene SNP marker” (#20700431); the Major Projects of Guizhou Province Science and Technology, “Guizhou Mountain Pasture Industrialization Production Technology Research and Application” (#6017; 2014); the Funding Project of 2016 Chongqing Universities Innovation Team Building Plan “Modern Technology in Beef Cattle Production”; Goat Industry Technology System Construction Project in Chongqing; and Development and Demonstration of Green Prevention and Control Technology of Plant Diseases and Pests in Grass Field System (#2014BAD23B03-03). REFERENCES Baum BR, Yen C, Yang JL, et al (1991). Roegneria: its generic limits and justification for its recognition. Can. J. Bot. 69: 282-294. http://dx.doi.org/10.1139/b91-038 Bhattacharyya P, Kumaria S, Kumar S, Tandon P, et al (2013). Start Codon Targeted (SCoT) marker reveals genetic diversity of Dendrobium nobile Lindl., an endangered medicinal orchid species. Gene 529: 21-26. http://dx.doi.org/10.1016/j.gene.2013.07.096 Chen DX, Li LY, Zhang X, Wang Y, et al (2015). Genetic structure and genetic diversity of single-variety Lonicera macranthoides populations in China, as indicated by SCoT markers. Genet. Mol. Res. 14: 8058-8067. http://dx.doi.org/10.4238/2015.July.17.14 Collard BCY, Mackill DJ, et al (2009). Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol. Biol. Report. 27: 86-93. http://dx.doi.org/10.1007/s11105-008-0060-5 Guo DL, Zhang JY, Liu CH, et al (2012). Genetic diversity in some grape varieties revealed by SCoT analyses. Mol. Biol. Rep. 39: 5307-5313. http://dx.doi.org/10.1007/s11033-011-1329-6 He Z, Liu Y, Chen L, Cao M, et al. (1998). Orthogonal design-direct analysis for PCR optimization. Hunan Yi Ke Da Xue Xue Bao 23: 403-404. Hu C, He XH, Luo C, Zhu JH, et al (2009). The optimization of SCoT-PCR system of Longan (Dimocarpus longan). Genom. Appl. Biol. 28: 970-974. Jiang DJ, Liu JM, et al (1990). Morphology and cytogenetics of intergeneric hybrid between Roegneria kamoji and Hordeum vulgare. Acta Genet. Sin. 17: 373-376. Joshi CP, Zhou H, Huang X, Chiang VL, et al (1997). Context sequences of translation initiation codon in plants. Plant Mol. Biol. 35: 993-1001. http://dx.doi.org/10.1023/A:1005816823636 Luo C, He XH, Chen H, Ou SJ, et al (2011). Genetic diversity of mango cultivars estimated using SCoT and ISSR markers. Biochem. Syst. Ecol. 39: 676-684. http://dx.doi.org/10.1016/j.bse.2011.05.023 Luo C, He XH, Hu Y, Yu HX, et al (2014). Oligo-dT anchored cDNA-SCoT: a novel differential display method for analyzing differential gene expression in response to several stress treatments in mango (Mangifera indica L.). Gene 548: 182-189. http://dx.doi.org/10.1016/j.gene.2014.07.024 Marson EP, Ferraz JB, Meirelles FV, Balieiro JC, et al (2005). Genetic characterization of European-Zebu composite bovine using RFLP markers. Genet. Mol. Res. 4: 496-505. Peng C, Liu QL, Wang HG, Yan XB, et al (2012). Optimization and testing for SSR-PCR system of Roegneria shandongensis. J. Biol. 29: 91-94. Xiao S, Zhang XQ, Ma X, Zhang JB, et al (2008). Genetic diversity of gliadin in wild germplasm of Roegneria kamoji. Acta Pratacul. Sin. 17: 138-144. Xiong F, Zhong R, Han Z, Jiang J, et al (2011). Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Mol. Biol. Rep. 38: 3487-3494. http://dx.doi.org/10.1007/s11033-010-0459-6 Yang RW, Zhou YH, Zheng YL, et al (2001). Analysis on chromosome C-banding of Roegneria kamoji. Guangxi Sci. 9: 138-141. Yang TT, Mu LQ, Wang J, et al (2007). Optimizing SSR-PCR system of Panax ginseng by orthogonal design. J. For. Res. 18: 31-34. http://dx.doi.org/10.1007/s11676-007-0006-z Yang ZJ, Peng ZS and Yang H (2016). Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.). Genet. Mol. Res. 15: gmr.15017509. Zeng B, Huang X, Huang LK, Zhang J, et al (2015). Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design. Genet. Mol. Res. 14: 3052-3061. http://dx.doi.org/10.4238/2015.April.10.15 Zhang Y, Zhou YH, Zhang L, Zhang HQ, et al (2005). Phylogenetic relationships among species of Roegneria, Elymus, Hystrix and Kengyilia (Poaceae: Triticeae) based on RAMP marker. Xibei Zhiwu Xuebao 25: 368-375. Zhang Y, Zhou YH, Zhang L, Zhang HQ, et al (2006). [PCR-RFLP analysis on Roegneria, Elymus, Hystrix and Kengyilia in Triticeae (Poaceae)]. Yi Chuan 28: 449-457. Zheng YQ, Wang ZY, Guo HL, Xue DD, et al (2008). Optimization of SRAP-PCR system on centipedegrass (Eremochloa ophiuroides) using orthogonal design and selection of primers. Acta Pratacul. Sin. 17: 110-117.
B. Zeng, Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., Mu, Q., Zeng, B., Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., Mu, Q., Zeng, B., Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., and Mu, Q., Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi, vol. 15, no. 4, p. -, 2016.
Conflicts of interest The authors declare no conflict of interest. ACKNOWLEDGMENTS Research supported by the Youth Project Foundation of Rongchang Campus, Southwest University, “The germplasm resources of Dactylis glomerata in rust resistance evaluation and development of rust-resistant gene SNP marker” (#20700431); the Major Projects of Guizhou Province Science and Technology, “Guizhou Mountain Pasture Industrialization Production Technology Research and Application” (#6017; 2014); the Funding Project of 2016 Chongqing Universities Innovation Team Building Plan “Modern Technology in Beef Cattle Production”; Goat Industry Technology System Construction Project in Chongqing; and Development and Demonstration of Green Prevention and Control Technology of Plant Diseases and Pests in Grass Field System (#2014BAD23B03-03). REFERENCES Baum BR, Yen C, Yang JL, et al (1991). Roegneria: its generic limits and justification for its recognition. Can. J. Bot. 69: 282-294. http://dx.doi.org/10.1139/b91-038 Bhattacharyya P, Kumaria S, Kumar S, Tandon P, et al (2013). Start Codon Targeted (SCoT) marker reveals genetic diversity of Dendrobium nobile Lindl., an endangered medicinal orchid species. Gene 529: 21-26. http://dx.doi.org/10.1016/j.gene.2013.07.096 Chen DX, Li LY, Zhang X, Wang Y, et al (2015). Genetic structure and genetic diversity of single-variety Lonicera macranthoides populations in China, as indicated by SCoT markers. Genet. Mol. Res. 14: 8058-8067. http://dx.doi.org/10.4238/2015.July.17.14 Collard BCY, Mackill DJ, et al (2009). Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol. Biol. Report. 27: 86-93. http://dx.doi.org/10.1007/s11105-008-0060-5 Guo DL, Zhang JY, Liu CH, et al (2012). Genetic diversity in some grape varieties revealed by SCoT analyses. Mol. Biol. Rep. 39: 5307-5313. http://dx.doi.org/10.1007/s11033-011-1329-6 He Z, Liu Y, Chen L, Cao M, et al. (1998). Orthogonal design-direct analysis for PCR optimization. Hunan Yi Ke Da Xue Xue Bao 23: 403-404. Hu C, He XH, Luo C, Zhu JH, et al (2009). The optimization of SCoT-PCR system of Longan (Dimocarpus longan). Genom. Appl. Biol. 28: 970-974. Jiang DJ, Liu JM, et al (1990). Morphology and cytogenetics of intergeneric hybrid between Roegneria kamoji and Hordeum vulgare. Acta Genet. Sin. 17: 373-376. Joshi CP, Zhou H, Huang X, Chiang VL, et al (1997). Context sequences of translation initiation codon in plants. Plant Mol. Biol. 35: 993-1001. http://dx.doi.org/10.1023/A:1005816823636 Luo C, He XH, Chen H, Ou SJ, et al (2011). Genetic diversity of mango cultivars estimated using SCoT and ISSR markers. Biochem. Syst. Ecol. 39: 676-684. http://dx.doi.org/10.1016/j.bse.2011.05.023 Luo C, He XH, Hu Y, Yu HX, et al (2014). Oligo-dT anchored cDNA-SCoT: a novel differential display method for analyzing differential gene expression in response to several stress treatments in mango (Mangifera indica L.). Gene 548: 182-189. http://dx.doi.org/10.1016/j.gene.2014.07.024 Marson EP, Ferraz JB, Meirelles FV, Balieiro JC, et al (2005). Genetic characterization of European-Zebu composite bovine using RFLP markers. Genet. Mol. Res. 4: 496-505. Peng C, Liu QL, Wang HG, Yan XB, et al (2012). Optimization and testing for SSR-PCR system of Roegneria shandongensis. J. Biol. 29: 91-94. Xiao S, Zhang XQ, Ma X, Zhang JB, et al (2008). Genetic diversity of gliadin in wild germplasm of Roegneria kamoji. Acta Pratacul. Sin. 17: 138-144. Xiong F, Zhong R, Han Z, Jiang J, et al (2011). Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Mol. Biol. Rep. 38: 3487-3494. http://dx.doi.org/10.1007/s11033-010-0459-6 Yang RW, Zhou YH, Zheng YL, et al (2001). Analysis on chromosome C-banding of Roegneria kamoji. Guangxi Sci. 9: 138-141. Yang TT, Mu LQ, Wang J, et al (2007). Optimizing SSR-PCR system of Panax ginseng by orthogonal design. J. For. Res. 18: 31-34. http://dx.doi.org/10.1007/s11676-007-0006-z Yang ZJ, Peng ZS and Yang H (2016). Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.). Genet. Mol. Res. 15: gmr.15017509. Zeng B, Huang X, Huang LK, Zhang J, et al (2015). Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design. Genet. Mol. Res. 14: 3052-3061. http://dx.doi.org/10.4238/2015.April.10.15 Zhang Y, Zhou YH, Zhang L, Zhang HQ, et al (2005). Phylogenetic relationships among species of Roegneria, Elymus, Hystrix and Kengyilia (Poaceae: Triticeae) based on RAMP marker. Xibei Zhiwu Xuebao 25: 368-375. Zhang Y, Zhou YH, Zhang L, Zhang HQ, et al (2006). [PCR-RFLP analysis on Roegneria, Elymus, Hystrix and Kengyilia in Triticeae (Poaceae)]. Yi Chuan 28: 449-457. Zheng YQ, Wang ZY, Guo HL, Xue DD, et al (2008). Optimization of SRAP-PCR system on centipedegrass (Eremochloa ophiuroides) using orthogonal design and selection of primers. Acta Pratacul. Sin. 17: 110-117.
B. Zeng, Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., Mu, Q., Zeng, B., Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., Mu, Q., Zeng, B., Yan, H. D., Huang, L. K., Wang, Y. C., Wu, J. H., Huang, X., Zhang, A. L., Wang, C. R., and Mu, Q., Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi, vol. 15, no. 4, p. -, 2016.
Conflicts of interest The authors declare no conflict of interest. ACKNOWLEDGMENTS Research supported by the Youth Project Foundation of Rongchang Campus, Southwest University, “The germplasm resources of Dactylis glomerata in rust resistance evaluation and development of rust-resistant gene SNP marker” (#20700431); the Major Projects of Guizhou Province Science and Technology, “Guizhou Mountain Pasture Industrialization Production Technology Research and Application” (#6017; 2014); the Funding Project of 2016 Chongqing Universities Innovation Team Building Plan “Modern Technology in Beef Cattle Production”; Goat Industry Technology System Construction Project in Chongqing; and Development and Demonstration of Green Prevention and Control Technology of Plant Diseases and Pests in Grass Field System (#2014BAD23B03-03). REFERENCES Baum BR, Yen C, Yang JL, et al (1991). Roegneria: its generic limits and justification for its recognition. Can. J. Bot. 69: 282-294. http://dx.doi.org/10.1139/b91-038 Bhattacharyya P, Kumaria S, Kumar S, Tandon P, et al (2013). Start Codon Targeted (SCoT) marker reveals genetic diversity of Dendrobium nobile Lindl., an endangered medicinal orchid species. Gene 529: 21-26. http://dx.doi.org/10.1016/j.gene.2013.07.096 Chen DX, Li LY, Zhang X, Wang Y, et al (2015). Genetic structure and genetic diversity of single-variety Lonicera macranthoides populations in China, as indicated by SCoT markers. Genet. Mol. Res. 14: 8058-8067. http://dx.doi.org/10.4238/2015.July.17.14 Collard BCY, Mackill DJ, et al (2009). Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol. Biol. Report. 27: 86-93. http://dx.doi.org/10.1007/s11105-008-0060-5 Guo DL, Zhang JY, Liu CH, et al (2012). Genetic diversity in some grape varieties revealed by SCoT analyses. Mol. Biol. Rep. 39: 5307-5313. http://dx.doi.org/10.1007/s11033-011-1329-6 He Z, Liu Y, Chen L, Cao M, et al. (1998). Orthogonal design-direct analysis for PCR optimization. Hunan Yi Ke Da Xue Xue Bao 23: 403-404. Hu C, He XH, Luo C, Zhu JH, et al (2009). The optimization of SCoT-PCR system of Longan (Dimocarpus longan). Genom. Appl. Biol. 28: 970-974. Jiang DJ, Liu JM, et al (1990). Morphology and cytogenetics of intergeneric hybrid between Roegneria kamoji and Hordeum vulgare. Acta Genet. Sin. 17: 373-376. Joshi CP, Zhou H, Huang X, Chiang VL, et al (1997). Context sequences of translation initiation codon in plants. Plant Mol. Biol. 35: 993-1001. http://dx.doi.org/10.1023/A:1005816823636 Luo C, He XH, Chen H, Ou SJ, et al (2011). Genetic diversity of mango cultivars estimated using SCoT and ISSR markers. Biochem. Syst. Ecol. 39: 676-684. http://dx.doi.org/10.1016/j.bse.2011.05.023 Luo C, He XH, Hu Y, Yu HX, et al (2014). Oligo-dT anchored cDNA-SCoT: a novel differential display method for analyzing differential gene expression in response to several stress treatments in mango (Mangifera indica L.). Gene 548: 182-189. http://dx.doi.org/10.1016/j.gene.2014.07.024 Marson EP, Ferraz JB, Meirelles FV, Balieiro JC, et al (2005). Genetic characterization of European-Zebu composite bovine using RFLP markers. Genet. Mol. Res. 4: 496-505. Peng C, Liu QL, Wang HG, Yan XB, et al (2012). Optimization and testing for SSR-PCR system of Roegneria shandongensis. J. Biol. 29: 91-94. Xiao S, Zhang XQ, Ma X, Zhang JB, et al (2008). Genetic diversity of gliadin in wild germplasm of Roegneria kamoji. Acta Pratacul. Sin. 17: 138-144. Xiong F, Zhong R, Han Z, Jiang J, et al (2011). Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Mol. Biol. Rep. 38: 3487-3494. http://dx.doi.org/10.1007/s11033-010-0459-6 Yang RW, Zhou YH, Zheng YL, et al (2001). Analysis on chromosome C-banding of Roegneria kamoji. Guangxi Sci. 9: 138-141. Yang TT, Mu LQ, Wang J, et al (2007). Optimizing SSR-PCR system of Panax ginseng by orthogonal design. J. For. Res. 18: 31-34. http://dx.doi.org/10.1007/s11676-007-0006-z Yang ZJ, Peng ZS and Yang H (2016). Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.). Genet. Mol. Res. 15: gmr.15017509. Zeng B, Huang X, Huang LK, Zhang J, et al (2015). Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design. Genet. Mol. Res. 14: 3052-3061. http://dx.doi.org/10.4238/2015.April.10.15 Zhang Y, Zhou YH, Zhang L, Zhang HQ, et al (2005). Phylogenetic relationships among species of Roegneria, Elymus, Hystrix and Kengyilia (Poaceae: Triticeae) based on RAMP marker. Xibei Zhiwu Xuebao 25: 368-375. Zhang Y, Zhou YH, Zhang L, Zhang HQ, et al (2006). [PCR-RFLP analysis on Roegneria, Elymus, Hystrix and Kengyilia in Triticeae (Poaceae)]. Yi Chuan 28: 449-457. Zheng YQ, Wang ZY, Guo HL, Xue DD, et al (2008). Optimization of SRAP-PCR system on centipedegrass (Eremochloa ophiuroides) using orthogonal design and selection of primers. Acta Pratacul. Sin. 17: 110-117.
2012
W. G. Xie, Lu, X. F., Zhang, X. Q., Huang, L. K., and Cheng, L., Genetic variation and comparison of orchardgrass (Dactylis glomerata L.) cultivars and wild accessions as revealed by SSR markers, vol. 11, pp. 425-433, 2012.
Benjamin EU, Kolliker R, Fujimori M and Komatsu T (2003). Genetic diversity in diploid cultivars of rhodesgrass determined on the basis of amplified fragment length polymorphism markers. Crop Sci. 43: 1516-1522. http://dx.doi.org/10.2135/cropsci2003.1516 Bolaric S, Barth S, Melchinger AE and Posselt UK (2005). Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers. Plant Breed. 124: 161-166. http://dx.doi.org/10.1111/j.1439-0523.2004.01032.x Charmet G and Balfourier F (1994). Isozyme variation and species relationships in the genus Lolium L. (Ryegrasses, Graminaceae). Theor. Appl. Genet. 87: 641-649. http://dx.doi.org/10.1007/BF00222888 Cheng ZP and Huang HW (2009). SSR fingerprinting Chinese peach cultivars and landraces (Prunus persica) and analysis of their genetic relationships. Sci. Hortic. 120: 188-193. http://dx.doi.org/10.1016/j.scienta.2008.10.008 Clunies-Ross T (1995). Mangolds, manure and mixtures. The importance of crop diversity on British farms. Ecologist 25: 181-187. Doyle JJ and Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13-15. Excoffier L, Smouse PE and Quattro JM (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479-491. PMid:1644282    PMCid:1205020 Fjellheim S and Rognli OA (2005). Genetic diversity within and among Nordic meadow fescue (Festuca pratensis Huds.) cultivars determined on the basis of AFLP markers. Crop Sci. 45: 2081-2086. http://dx.doi.org/10.2135/cropsci2005.0091 Hamrick JL and Godt MJW (1990). Allozyme Diversity in Plant Species. In: Plant Population Genetics, Breeding, and Genetic Resources (Brown AHD, Clegg MT and Kahler AL, eds.). Sinauer, Sunderland, 43-63. Larson SR, Jones TA and Jensen KB (2004). Population structure in Pseudoroegneria spicata (Poaceae: Triticeae) modeled by Bayesian clustering of AFLP genotypes. Am. J. Bot. 91: 1789-1801. http://dx.doi.org/10.3732/ajb.91.11.1789 PMid:21652326 Nei M (1973). Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. U. S. A. 70: 3321-3323. http://dx.doi.org/10.1073/pnas.70.12.3321 Peng Y, Zhang X, Deng Y and Ma X (2008). Evaluation of genetic diversity in wild orchardgrass (Dactylis glomerata L.) based on AFLP markers. Hereditas 145: 174-181. http://dx.doi.org/10.1111/j.0018-0661.2008.02038.x Rohlf FJ (1997). NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, Ver. 108. Applied Biostatistics Inc., New York. Tahan O, Geng Y, Zeng L, Dong S, et al. (2009). Assessment of genetic diversity and population structure of Chinese wild almond, Amygdalus nana, using EST- and genomic-SSRs. Biochem. Syst. Ecol. 37: 146-153. http://dx.doi.org/10.1016/j.bse.2009.02.006 Xie WG, Zhang XQ, Cai HW, Liu W, et al. (2010). Genetic diversity analysis and transferability of cereal EST-SSR markers to orchardgrass (Dactylis glomerata L.). Biochem. Syst. Ecol. 38: 740-749. http://dx.doi.org/10.1016/j.bse.2010.06.009 Yeh FC, Yang RC and Boyle T (1999). POPGENE ver. 1.32. Microsoft Windows-Based Freeware for Population Genetic Analysis. Quick User Guide. Center Int. For. Res. University of Alberta, Edmonton. Zeng B, Zhang XQ, Fan Y, Lan Y, et al. (2006). Genetic diversity of Dactylis glomerata germplasm resources detected by inter-simple sequence repeats (ISSRS) molecular markers. Yi. Chuan 28: 1093-1100. http://dx.doi.org/10.1360/yc-006-1093 PMid:16963418 Zeng B, Zhang XQ and Lan Y (2008). Evaluation of genetic diversity and relationships in orchardgrass (Dactylis glomerata L.) germplasm based on SRAP markers. Can. J. Plant Sci. 88: 53-60. http://dx.doi.org/10.4141/CJPS07017 Zhang FM (2001). DCFA 1.1, a Program Companied with AMOVA to Compute the Matrix of Distance. Laboratory of Systematics and Evolutionary Botany, Institute of Botany. The Chinese Academy of Sciences, Beijing. Zoghlami N, Riahi L, Laucou V, Lacombe T, et al. (2009). Origin and genetic diversity of Tunisian grapes as revealed by microsatellite markers. Sci. Hortic. 120: 479-486. http://dx.doi.org/10.1016/j.scienta.2008.12.011