Review

Related GMR Articles

11/18/2013
Isolation; Plant RNA; Polyphenols; Polysaccharides; RNA-seq

A recently developed revolutionary approach to transcrip­tomics, RNA-Seq, and suppression subtractive hybridization are power­ful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that not only limits the application ... more

Y.Q. Zhu; W.J. Wu; H.W. Xiao; H.B. Chen; Y. Zheng; Y.J. Zhang; H. .X.Wang; L.Q. Huang
10/07/2014
Differential expression genes; Metastatic melanoma; RNA-seq; Single nucleotide variations

Melanoma is a malignant tumor and one of the most frequent metastatic cancers. This study was conducted to identify differential expression genes (DEGs) and single nucleotide variations (SNVs) in metastatic melanoma. We analyzed microarray data of GSE23056 downloaded from the Gene Expression Omnibus, including two normal samples (skinN1 and skinN2) and 2 metastatic melanoma samples (skinT ... more

D. Liu; Z.G. Zhao; Z.L. Jiao; H.J. Li
03/26/2014
Growth inhibition; Low-energy ion-beam implantation; Photosynthesis; RNA-seq; Transcriptome

Transcriptomic research based on RNA sequencing (RNA-seq) technology is innovative and will provide new opportunities and biological perspectives for the use of ion-beam implantation in plants. Using RNA-seq, transcriptomes of whole rice seedlings generated from seeds implanted with a low-energy N+ beam were analyzed 96 h after planting. We identified 544 transcripts that were ... more

Q.F. Chen; H.Y. Ya; W.D. Wang; Z. Jiao
06/01/2015
Chinese bayberry; EST-SSR; Myrica rubra; Polymorphism; RNA-seq

Most plant expressed sequence tag-simple sequence repeats (EST-SSRs) are not polymorphic, and it is important to learn the characteristics of highly polymorphic EST-SSRs. In this study, 357 compound and 5557 non-compound EST-SSRs, identified from the transcriptome of the Chinese bayberry (Myrica rubra ‘Biqi’), were divided into 11 types based on their characteristics. ... more

S.Y. Zhang; C. Feng; C.J. Xu; C.Q. Zhu; K.S. Chen
09/23/2013
Anticytotoxicity; Antigenotoxicity; Bacteria; Lafoensia pacari; Mice; Pacari

Lafoensia pacari (Lythraceae), popularly known in Brazil as "pacari", is a small tree native to the Cerrado that is used in folk medicine to treat cancer and as an anti-inflammatory and cicatrizing agent. We evaluated the genotoxic, cytotoxic, antigenotoxic, and anticytotoxic activities of an ethanol extract of L. pacari stem bark (EESB) using the Ames test and the mouse ... more

D.C.S. Lima; C.R. Silva; B.L. Sampaio; J.R. de Paula; L. Chen-Chen
09/10/2013
Bacteria; Carboxymethyl cellulose; Digestive enzymes; Microcrystalline cellulose; Saproxylic larvae; Xylan

We analyzed the digestive activity of the enzymes that digest cellulose and hemicellulose and the bacterial community that is capable of hydrolyzing wood compounds in the digestive tracts of Stenochironomus (Diptera: Chironomidae) larvae, which are miners of decomposing submerged tree and bush branches. Based on quantification of reducing sugars, these larvae have a limited ... more

R. Koroiva; C.W.O. Souza; D. Toyama; F. Henrique-Silva; A.A. Fonseca-Gessner
01/22/2014
Antibiotics; Bacteria; Burns; Drug resistance

This study aimed to analyze the spectrum and drug resistance of bacteria isolated from burn patients to provide a reference for rational clinical use of antibiotics. Up to 1914 bacterial strain specimens isolated from burn patients admitted to hospital between 2001 and 2010 were subjected to resistance monitoring by using the K-B paper disk method. Retrospective analysis was performed on ... more

L.F. Wang; J.L. Li; W.H. Ma; J.Y. Li
06/17/2014
Bacteria; Compositional features; Essential gene prediction; Library for support vector machine

Essential genes are those genes that are needed by organisms at any time and under any conditions. It is very important for us to identify essential genes from bacterial genomes because of their vital role in synthetic biology and biomedical practices. In this paper, we developed a support vector machine (SVM)-based method to predict essential genes of bacterial genomes using only ... more

L.W. Ning; H. Lin; H. Ding; J. Huang; N. Rao; F.B. Guo